Fifty-seven genital tracts of regularly slaughtered culled Piedmontese cows, aged 7.4 ± 4.3 years (mean ± SD), range: 2.6-15.6 years, were grossly and microscopically examined. DNA extracted from oviducts was subjected to PCR to evaluate the presence of Chlamydia spp. The 15 PCR-positive oviducts were subjected to Sanger sequencing and showed the presence of Chamydia abortus, with an identity range between 99 and 100%. Nine of the PCR-positive samples belonged to the 24 animals with a normal macroscopic appearance of the whole genital tract (percentage of positive oviducts in normal genital tracts 9/24 = 37.5%), while six belonged to the 33 genital tracts with lesions in one or more organs (percentage of positive oviducts in pathological genital tracts 6/33 = 18.1%); of these, a single animal had salpingitis. The detection of C. abortus in bovine oviducts is of particular interest because it has never been previously investigated or reported

Chlamydia abortus in cows oviducts, occasional event or casula connection? / Appino, S; Vincenti, L; Rota, A; Pellegrini, S; Chieppa, Mn; Cadoni, V; Pregel, P.. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 1439-0531. - (2015). [10.1111/rda.12505]

Chlamydia abortus in cows oviducts, occasional event or casula connection?

Appino S
;
2015

Abstract

Fifty-seven genital tracts of regularly slaughtered culled Piedmontese cows, aged 7.4 ± 4.3 years (mean ± SD), range: 2.6-15.6 years, were grossly and microscopically examined. DNA extracted from oviducts was subjected to PCR to evaluate the presence of Chlamydia spp. The 15 PCR-positive oviducts were subjected to Sanger sequencing and showed the presence of Chamydia abortus, with an identity range between 99 and 100%. Nine of the PCR-positive samples belonged to the 24 animals with a normal macroscopic appearance of the whole genital tract (percentage of positive oviducts in normal genital tracts 9/24 = 37.5%), while six belonged to the 33 genital tracts with lesions in one or more organs (percentage of positive oviducts in pathological genital tracts 6/33 = 18.1%); of these, a single animal had salpingitis. The detection of C. abortus in bovine oviducts is of particular interest because it has never been previously investigated or reported
Chlamydia abortus in cows oviducts, occasional event or casula connection? / Appino, S; Vincenti, L; Rota, A; Pellegrini, S; Chieppa, Mn; Cadoni, V; Pregel, P.. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 1439-0531. - (2015). [10.1111/rda.12505]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/203206
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