Design of Amperometric Biosensors for the Detection of Glucose Prepared by Immobilization of Glucose Oxidase on Conducting (Poly)Thiophene Films

Enzyme-based sensors have emerged as important analytical tools with application in diverse 1elds, and biosensors for the detection of glucose using the enzyme glucose oxidase have been widely investigated. In this work, the preparation of biosensors by electrochemical polymerization of (poly)thiophenes, namely 2,2′-bithiophene (2,2′-BT) and 4,4′-bis(2-methyl-3-butyn-2-ol)-2,2′- bithiophene (4,4′-bBT), followed by immobilization of glucose oxidase on the 1lms, is described. N-cyclohexyl-N′-(2-morpholinoethyl) carbodiimide metho-p-toluenesulfonate (CMC) was used as a condensing agent, and p-benzoquinone (BQ) was used as a redox mediator in solution. -e glucose oxidase electrodes with 1lms of 2,2′-BT and 4,4′-bBT were then tested for their ability in detecting glucose from synthetic and real samples (pear, apricot, and peach fruit juices).