A cellular, α-Amanitin sensitive, RNA polymerase from mouse L cells binds “in vitro” to Mengovirus-induced, double-stranded (ds) RNA. Formation of the “enzyme-ds RNA” complex was studied by the nitrocellulose filter technique. Reaction strongly depends on K+/(NH)++ and Mn++/Mg++ concentration, occurs optimally at 37°C, is linear with time up to 10 minutes, and is inhibited by a rifamycin derivative AF/013. Competition experiments demonstrated that neither heat-denatured Mengovirus RF nor single-stranded ribonucleotides interfere with complex formation, whereas the double-helical form same RNAs (native RF, Poly I:C; Poly A:U) efficiently compete with Mengovirus RF for cellular polymerase. These results seem to indicate that the double-helical nature of the template is essential for binding to occur.

"In vitro" binding of cellular, alpha-amanitin sensitive, RNA polymerase to infectious, mengovirus-induced double-stranded RNA / Perez-Bercoff, R.; Carrara, G.; Dolei, A.; Conciatori, G.; Rita, G.. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 56:4(1974), pp. 876-883. [10.1016/S0006-291X(74)80270-6]

"In vitro" binding of cellular, alpha-amanitin sensitive, RNA polymerase to infectious, mengovirus-induced double-stranded RNA

A. Dolei;
1974

Abstract

A cellular, α-Amanitin sensitive, RNA polymerase from mouse L cells binds “in vitro” to Mengovirus-induced, double-stranded (ds) RNA. Formation of the “enzyme-ds RNA” complex was studied by the nitrocellulose filter technique. Reaction strongly depends on K+/(NH)++ and Mn++/Mg++ concentration, occurs optimally at 37°C, is linear with time up to 10 minutes, and is inhibited by a rifamycin derivative AF/013. Competition experiments demonstrated that neither heat-denatured Mengovirus RF nor single-stranded ribonucleotides interfere with complex formation, whereas the double-helical form same RNAs (native RF, Poly I:C; Poly A:U) efficiently compete with Mengovirus RF for cellular polymerase. These results seem to indicate that the double-helical nature of the template is essential for binding to occur.
"In vitro" binding of cellular, alpha-amanitin sensitive, RNA polymerase to infectious, mengovirus-induced double-stranded RNA / Perez-Bercoff, R.; Carrara, G.; Dolei, A.; Conciatori, G.; Rita, G.. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 56:4(1974), pp. 876-883. [10.1016/S0006-291X(74)80270-6]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/202225
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? ND
social impact