Micro-RNA (miRNA) are a family of small non-coding ribonucleic acids that inhibits post-transcriptionally the expression of their target messenger RNA (mRNA). We are interested in studying the involvement of miRNA in longevity and autoimmune diseases. In this study we compared the different expression of seven microRNAs between human plasma healthy controls, plasma samples of centenarians and samples from patients with rheumatoid arthritis. We used the Life Technologies' protocol to quantify seven miRNAs from 62 plasma samples: 20 healthy human controls, 14 centenarians, 28 patients with rheumatoid arthritis. TaqMan MicroRNA assays were used to analyze the expression profiles of miR-125b-5p, miR-425-5p, miR-200b5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-21-5p and miR-126-3p. The relative expression of mature miRNAs was analyzed using software REST. Our results show that miR-425-5p, miR-21 and miR-212 significantly decreased in centenarians and in patients with rheumatoid arthritis compared with controls. Furthermore in this work we highlight a connection between corticosteroid treatment and miRNAs expression.

MicroRNA expression analysis of centenarians and rheumatoid arthritis patients reveals a common expression pattern / Balzano, Francesca; Deiana, Marta; Dei Giudici, Silvia; Oggiano, Annalisa; Pasella, Sara; Pinna, Sara; Mannu, Andrea; Deiana, Nicola; Porcu, Baingio; Masala, Antonio Giovanni Emilio; Pileri, Piera V.; Scognamillo, Fabrizio; Pala, Carlo; Zinellu, Angelo; Carru, Ciriaco; Deiana, Luca. - In: INTERNATIONAL JOURNAL OF MEDICAL SCIENCES. - ISSN 1449-1907. - 14:7(2017), pp. 622-628. [10.7150/ijms.18972]

MicroRNA expression analysis of centenarians and rheumatoid arthritis patients reveals a common expression pattern

BALZANO, Francesca;DEIANA, Marta;Oggiano, Annalisa;PASELLA, Sara;PINNA, Sara;MANNU, Andrea;DEIANA, Nicola;PORCU, Baingio;MASALA, Antonio Giovanni Emilio;SCOGNAMILLO, Fabrizio;PALA, Carlo;ZINELLU, Angelo;CARRU, Ciriaco;DEIANA, Luca
2017

Abstract

Micro-RNA (miRNA) are a family of small non-coding ribonucleic acids that inhibits post-transcriptionally the expression of their target messenger RNA (mRNA). We are interested in studying the involvement of miRNA in longevity and autoimmune diseases. In this study we compared the different expression of seven microRNAs between human plasma healthy controls, plasma samples of centenarians and samples from patients with rheumatoid arthritis. We used the Life Technologies' protocol to quantify seven miRNAs from 62 plasma samples: 20 healthy human controls, 14 centenarians, 28 patients with rheumatoid arthritis. TaqMan MicroRNA assays were used to analyze the expression profiles of miR-125b-5p, miR-425-5p, miR-200b5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-21-5p and miR-126-3p. The relative expression of mature miRNAs was analyzed using software REST. Our results show that miR-425-5p, miR-21 and miR-212 significantly decreased in centenarians and in patients with rheumatoid arthritis compared with controls. Furthermore in this work we highlight a connection between corticosteroid treatment and miRNAs expression.
MicroRNA expression analysis of centenarians and rheumatoid arthritis patients reveals a common expression pattern / Balzano, Francesca; Deiana, Marta; Dei Giudici, Silvia; Oggiano, Annalisa; Pasella, Sara; Pinna, Sara; Mannu, Andrea; Deiana, Nicola; Porcu, Baingio; Masala, Antonio Giovanni Emilio; Pileri, Piera V.; Scognamillo, Fabrizio; Pala, Carlo; Zinellu, Angelo; Carru, Ciriaco; Deiana, Luca. - In: INTERNATIONAL JOURNAL OF MEDICAL SCIENCES. - ISSN 1449-1907. - 14:7(2017), pp. 622-628. [10.7150/ijms.18972]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/181443
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