Outer spore envelope proteins of pathogenic bacteria often present specific virulence factors and tools to evade the defence system of their hosts. Brevibacillus laterosporus, a pathogen of invertebrates and an antimicrobial-producing species, is characterised by a unique spore coat and canoe-shaped parasporal body (SC-CSPB) complex surrounding the core spore. In the present study, we identified and characterised major proteins of the SC-CSPB complex of B. laterosporus, and we investigated their entomopathogenic role. Employing a proteomic approach and a B. laterosporus-house fly study model, we found four highly conserved proteins (ExsC, CHRD, CpbA and CpbB) that function as insect virulence factors. CpbA was associated with a significantly higher mortality of flies and greater relative gene expression levels during sporulation, compared to the other SC-CSPB proteins. Taken together, we suggest that spore surface proteins are a part of a complex set of toxins and virulence factors that B. laterosporus employs in its pathogenicity against flies.

Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis / Marche, Maria Giovanna; Mura, Maria Elena; Falchi, Giovanni; Ruiu, Luca. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7:(2017), p. 43805. [10.1038/srep43805]

Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis

MARCHE, Maria Giovanna;MURA, Maria Elena;FALCHI, Giovanni;RUIU, Luca
2017-01-01

Abstract

Outer spore envelope proteins of pathogenic bacteria often present specific virulence factors and tools to evade the defence system of their hosts. Brevibacillus laterosporus, a pathogen of invertebrates and an antimicrobial-producing species, is characterised by a unique spore coat and canoe-shaped parasporal body (SC-CSPB) complex surrounding the core spore. In the present study, we identified and characterised major proteins of the SC-CSPB complex of B. laterosporus, and we investigated their entomopathogenic role. Employing a proteomic approach and a B. laterosporus-house fly study model, we found four highly conserved proteins (ExsC, CHRD, CpbA and CpbB) that function as insect virulence factors. CpbA was associated with a significantly higher mortality of flies and greater relative gene expression levels during sporulation, compared to the other SC-CSPB proteins. Taken together, we suggest that spore surface proteins are a part of a complex set of toxins and virulence factors that B. laterosporus employs in its pathogenicity against flies.
2017
Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis / Marche, Maria Giovanna; Mura, Maria Elena; Falchi, Giovanni; Ruiu, Luca. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7:(2017), p. 43805. [10.1038/srep43805]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/176602
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