The tetradecapeptide containing the 10 aminoacid repeated sequence on the C-terminus of the Ni(II)-induced Cap43 protein, was analyzed for Ni(II) and Cu(II) binding. A combined pH-metric and spectroscopic UV-VIS, EPR, CD and NMR study of Ni(II) and Cu(II) binding to the blocked CH3CO-Thr-Arg-Ser-Arg-Ser-His-Thr-Ser-Glu-Gly-Thr-Arg-Ser-Arg-NH2 (Ac-TRSRSHTSEGTRSR-Am) peptide, modeling a part of the C-terminal sequence of the Cap43 protein, revealed the formation of octahedral complexes involving imidazole nitrogen of histidine, at pH 5.5 and pH 7 for Cu(II) and Ni(II), respectively; a major square planar 4N-Ni(II) complex (about 100% at pH 9, log K*=-28.16) involving imidazole nitrogen of histidine and three deprotonated amide nitrogens of the backbone of the peptide was revealed; a 3N-Cu(II) complex (maximum about 70% at pH 7, log K*=-13.91) and a series of 4N-Cu(II) complexes starting at pH 5.5 (maximum about 90% at pH 8.7, log K*=-21.39 for CuH-3L), were revealed. This work supports the existence of a metal binding site at the COOH-terminal part of the Cap43 peptide. Copyright © 2001 Elsevier Science B.V.

Ni(II) and Cu(II) Binding with a 14-aminoacid Sequence of Cap43 protein, TRSRSHTSEGTRSR / Zoroddu, Maria Antonietta; Kowalik Jankowska, Teresa; Kozlowski, Henryk; Salnikow, Konstantin; Costa, Max. - In: JOURNAL OF INORGANIC BIOCHEMISTRY. - ISSN 0162-0134. - 84:1-2(2001), pp. 47-54. [10.1016/S0162-0134(00)00204-X]

Ni(II) and Cu(II) Binding with a 14-aminoacid Sequence of Cap43 protein, TRSRSHTSEGTRSR

ZORODDU, Maria Antonietta;
2001-01-01

Abstract

The tetradecapeptide containing the 10 aminoacid repeated sequence on the C-terminus of the Ni(II)-induced Cap43 protein, was analyzed for Ni(II) and Cu(II) binding. A combined pH-metric and spectroscopic UV-VIS, EPR, CD and NMR study of Ni(II) and Cu(II) binding to the blocked CH3CO-Thr-Arg-Ser-Arg-Ser-His-Thr-Ser-Glu-Gly-Thr-Arg-Ser-Arg-NH2 (Ac-TRSRSHTSEGTRSR-Am) peptide, modeling a part of the C-terminal sequence of the Cap43 protein, revealed the formation of octahedral complexes involving imidazole nitrogen of histidine, at pH 5.5 and pH 7 for Cu(II) and Ni(II), respectively; a major square planar 4N-Ni(II) complex (about 100% at pH 9, log K*=-28.16) involving imidazole nitrogen of histidine and three deprotonated amide nitrogens of the backbone of the peptide was revealed; a 3N-Cu(II) complex (maximum about 70% at pH 7, log K*=-13.91) and a series of 4N-Cu(II) complexes starting at pH 5.5 (maximum about 90% at pH 8.7, log K*=-21.39 for CuH-3L), were revealed. This work supports the existence of a metal binding site at the COOH-terminal part of the Cap43 peptide. Copyright © 2001 Elsevier Science B.V.
2001
Ni(II) and Cu(II) Binding with a 14-aminoacid Sequence of Cap43 protein, TRSRSHTSEGTRSR / Zoroddu, Maria Antonietta; Kowalik Jankowska, Teresa; Kozlowski, Henryk; Salnikow, Konstantin; Costa, Max. - In: JOURNAL OF INORGANIC BIOCHEMISTRY. - ISSN 0162-0134. - 84:1-2(2001), pp. 47-54. [10.1016/S0162-0134(00)00204-X]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/166747
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