Several works have demonstrated the existence of a link between Mycobacterium avium subsp. Paratuberculosis (MAP) and MS in Italy. In this study, we analyzed the serology of MAP in a Japanese population while looking at several markers of MAP. Fifty MS patients, 12 clinically isolated syndrome (CIS) patients, 30 other neurological disorders (OND) patients, and 50 healthy controls (HCs) were tested using ELISA for the presence of IgG antibodies toward immunodominant epitopes MAP-0106c121-132, homologues MBP85-98, homologues IRF5424-432, MAP-402718-32, and MAP-2694295-303. MAP-positive patients were also analyzed in relation to their clinical/demographic characteristics. Amongst all peptides, only antibodies against MAP-2694295-303 were more prevalent in MS patients (30%), as compared to OND patients (3%) (p = 0.009; area under roc curve (AUC) = 0.61) and HCs (2%) (p = 0.0004; AUC = 0.65) and in CIS patients (25%) compared to HCs (p = 0.023; AUC = 0.55). Logistic regression analysis showed a higher frequency of anti-MAP-2694295-303 antibodies in the sera of oligoclonal bands positive MS patients (p = 0.2; OR = 2, 95%CI: 0.55-7.7). These findings support the view that MAP could act as a risk factor or a triggering agent of MS in some Japanese patients with a genetic susceptibility to the mycobacterium.

Humoral response against host-mimetic homologous epitopes of Mycobacterium avium subsp. Paratuberculosis in Japanese multiple sclerosis patients / Cossu, Davide; Yokoyama, Kazumasa; Sechi, Leonardo Antonio; Otsubo, Shigeru; Tomizawa, Yuji; Momotani, Eiichi; Hattori, Nobutaka. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 6:(2016), p. 29227. [10.1038/srep29227]

Humoral response against host-mimetic homologous epitopes of Mycobacterium avium subsp. Paratuberculosis in Japanese multiple sclerosis patients

COSSU, Davide;SECHI, Leonardo Antonio;
2016-01-01

Abstract

Several works have demonstrated the existence of a link between Mycobacterium avium subsp. Paratuberculosis (MAP) and MS in Italy. In this study, we analyzed the serology of MAP in a Japanese population while looking at several markers of MAP. Fifty MS patients, 12 clinically isolated syndrome (CIS) patients, 30 other neurological disorders (OND) patients, and 50 healthy controls (HCs) were tested using ELISA for the presence of IgG antibodies toward immunodominant epitopes MAP-0106c121-132, homologues MBP85-98, homologues IRF5424-432, MAP-402718-32, and MAP-2694295-303. MAP-positive patients were also analyzed in relation to their clinical/demographic characteristics. Amongst all peptides, only antibodies against MAP-2694295-303 were more prevalent in MS patients (30%), as compared to OND patients (3%) (p = 0.009; area under roc curve (AUC) = 0.61) and HCs (2%) (p = 0.0004; AUC = 0.65) and in CIS patients (25%) compared to HCs (p = 0.023; AUC = 0.55). Logistic regression analysis showed a higher frequency of anti-MAP-2694295-303 antibodies in the sera of oligoclonal bands positive MS patients (p = 0.2; OR = 2, 95%CI: 0.55-7.7). These findings support the view that MAP could act as a risk factor or a triggering agent of MS in some Japanese patients with a genetic susceptibility to the mycobacterium.
2016
Humoral response against host-mimetic homologous epitopes of Mycobacterium avium subsp. Paratuberculosis in Japanese multiple sclerosis patients / Cossu, Davide; Yokoyama, Kazumasa; Sechi, Leonardo Antonio; Otsubo, Shigeru; Tomizawa, Yuji; Momotani, Eiichi; Hattori, Nobutaka. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 6:(2016), p. 29227. [10.1038/srep29227]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/162339
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 18
social impact