The order Proseriata (Platyhelminthes) represents one of the most abundant soft-bodied meiofaunal groups. These minute interstitial organisms are characterized by a very simple morphology, which often hides the occurrence of cryptic species. Accordingly, molecular analyses are often needed to provide reliable taxonomic assessment and/or species identification. For these purposes the 18S and 28S rDNA genes have been so far the markers of choice. Here, we present a set of new primers for the sequencing of the whole ribosomal region in Proseriata, which improves the size of the sequenced segment from the current 3100 bp (18S ? 28S D1–D6) to about 6300 bp. A broader molecular dataset may be used to achieve deeper insights on the systematics of Proseriata.
New set of nuclear primers for the ribosomal regions in Proseriata (Platyhelminthes) / Scarpa, Fabio; Sanna, Daria; Lai, Tiziana; Cossu, Piero; CURINI GALLETTI, Marco; Casu, Marco. - In: CONSERVATION GENETICS RESOURCES. - ISSN 1877-7252. - (2016). [10.1007/s12686-016-0583-2]