The order Proseriata (Platyhelminthes) represents one of the most abundant soft-bodied meiofaunal groups. These minute interstitial organisms are characterized by a very simple morphology, which often hides the occurrence of cryptic species. Accordingly, molecular analyses are often needed to provide reliable taxonomic assessment and/or species identification. For these purposes the 18S and 28S rDNA genes have been so far the markers of choice. Here, we present a set of new primers for the sequencing of the whole ribosomal region in Proseriata, which improves the size of the sequenced segment from the current 3100 bp (18S ? 28S D1–D6) to about 6300 bp. A broader molecular dataset may be used to achieve deeper insights on the systematics of Proseriata.

New set of nuclear primers for the ribosomal regions in Proseriata (Platyhelminthes) / Scarpa, Fabio; Sanna, Daria; Lai, Tiziana; Cossu, Piero; Curini Galletti, Marco; Casu, Marco. - In: CONSERVATION GENETICS RESOURCES. - ISSN 1877-7252. - (2016). [10.1007/s12686-016-0583-2]

New set of nuclear primers for the ribosomal regions in Proseriata (Platyhelminthes)

SCARPA, Fabio;SANNA, Daria;LAI, Tiziana;COSSU, Piero;CURINI GALLETTI, Marco;CASU, Marco
2016

Abstract

The order Proseriata (Platyhelminthes) represents one of the most abundant soft-bodied meiofaunal groups. These minute interstitial organisms are characterized by a very simple morphology, which often hides the occurrence of cryptic species. Accordingly, molecular analyses are often needed to provide reliable taxonomic assessment and/or species identification. For these purposes the 18S and 28S rDNA genes have been so far the markers of choice. Here, we present a set of new primers for the sequencing of the whole ribosomal region in Proseriata, which improves the size of the sequenced segment from the current 3100 bp (18S ? 28S D1–D6) to about 6300 bp. A broader molecular dataset may be used to achieve deeper insights on the systematics of Proseriata.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/160801
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