In reptiles,administrationofopioiddrugshasyieldedunexpectedresultswithrespecttoanalgesia. Tapentadol(TAP)isanovelatypicalopioiddruglabelledforhumanuse.Theobjectiveofthisstudywas to evaluatethepharmacokineticsandthepharmacodynamicsofthisdruginyellow-belliedsliders,aftera single intramuscular(IM)injectionof5mg/kgofTAP.Turtles(n ¼ 9) wererandomlyassignedto2 treatment groups,accordingtoasingle-dose,single-treatment,unpaired,2-periodcrossoverdesign. Group A(n ¼ 5) receivedasingleIM(proximalfrontlimb)doseofTAP(5mg/mL)at5mg/kg.GroupB (n ¼ 4) receivedasingleIMinjectionofsaline(equivalentvolumetoopioidvolumes)ofTAPatthesame site. Aftera1-monthwashoutperiod,groupswererotatedandtheexperimentrepeated.TAPplasma concentrationsweredeterminedbyavalidatedhigh-performanceliquidchromatography-fluorescence method, andaninfraredthermalstimuliwasappliedtotheplantarsurfaceoftheturtles’ hind limbsto evaluatethethermalwithdrawallatency(TWL).TAPplasmaconcentrationsweredetectablebetween1 and 24hour(s)(1619and37ng/mL,respectively).TheTAP-treatedgroupshowedanincreaseinTWL1 hour afterdrugadministration(13.32 6.40 seconds).Subsequently,TWLdecreasedwithtimeand significant differencesbetweentreatmentandcontrolgroupswereapparentupto10hoursfollowing treatment. Alinearrelationship(r2 ¼ 0.99)betweenTAPplasmaconcentrationandeffectwasfound. Given these findings,TAPappearstobeanattractiveoptionforantinociceptioninturtles,owingtoits rapid onsetandacceptabledurationofeffect.
PHARMACOKINETICANDPHARMACODYNAMIC ASSESSMENTSOFTAPENTADOLINYELLOW-BELLIED SLIDER TURTLES(TRACHEMYS SCRIPTASCRIPTA) AFTER ASINGLEINTRAMUSCULARINJECTION / Giorgi, M; Lee HK Rota, S; Owen, H; Vito, De; Virginia, ; Demontis, Maria Piera; Varoni, Maria Vittoria. - In: JOURNAL OF EXOTIC PET MEDICINE. - ISSN 1557-5063. - 24:(2015), pp. 317-325. [10.1053/j.jepm.2015.06.004]
PHARMACOKINETICANDPHARMACODYNAMIC ASSESSMENTSOFTAPENTADOLINYELLOW-BELLIED SLIDER TURTLES(TRACHEMYS SCRIPTASCRIPTA) AFTER ASINGLEINTRAMUSCULARINJECTION
Giorgi M;DE VITO;DEMONTIS Maria Piera;VARONI Maria Vittoria
2015-01-01
Abstract
In reptiles,administrationofopioiddrugshasyieldedunexpectedresultswithrespecttoanalgesia. Tapentadol(TAP)isanovelatypicalopioiddruglabelledforhumanuse.Theobjectiveofthisstudywas to evaluatethepharmacokineticsandthepharmacodynamicsofthisdruginyellow-belliedsliders,aftera single intramuscular(IM)injectionof5mg/kgofTAP.Turtles(n ¼ 9) wererandomlyassignedto2 treatment groups,accordingtoasingle-dose,single-treatment,unpaired,2-periodcrossoverdesign. Group A(n ¼ 5) receivedasingleIM(proximalfrontlimb)doseofTAP(5mg/mL)at5mg/kg.GroupB (n ¼ 4) receivedasingleIMinjectionofsaline(equivalentvolumetoopioidvolumes)ofTAPatthesame site. Aftera1-monthwashoutperiod,groupswererotatedandtheexperimentrepeated.TAPplasma concentrationsweredeterminedbyavalidatedhigh-performanceliquidchromatography-fluorescence method, andaninfraredthermalstimuliwasappliedtotheplantarsurfaceoftheturtles’ hind limbsto evaluatethethermalwithdrawallatency(TWL).TAPplasmaconcentrationsweredetectablebetween1 and 24hour(s)(1619and37ng/mL,respectively).TheTAP-treatedgroupshowedanincreaseinTWL1 hour afterdrugadministration(13.32 6.40 seconds).Subsequently,TWLdecreasedwithtimeand significant differencesbetweentreatmentandcontrolgroupswereapparentupto10hoursfollowing treatment. Alinearrelationship(r2 ¼ 0.99)betweenTAPplasmaconcentrationandeffectwasfound. Given these findings,TAPappearstobeanattractiveoptionforantinociceptioninturtles,owingtoits rapid onsetandacceptabledurationofeffect.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
