Milk fat globules (MFGs) are secretory vesicles assembled and secreted by mammary epithelial cells during lactation. They consist of fat globules surrounded by a lipid bilayer membrane which is derived from the apical membrane of the lactating cells. MFGs contain, besides lipids, proteins from the apical plasma membrane and from the cytoplasmatic material. Their peculiar vesicle nature makes them a suitable and easily available source of biological material in monitoring the physiopathological state of the mammary gland. Unfortunately, the conspicuous lipidic component of MFGs consistently limits protein extraction and purification for MFG proteomic investigations. This work deals with the development of a suitable procedure for
Solubilization methods and reference 2-DE map of cow milk fat globules / Bianchi, L; Puglia, M; Landi, C; Matteoni, S; Perini, D; Armini, A; Verani, M; Trombetta, C; Soldani, P; Roncada, P; Greppi, Gianfranco; Pallini, V; Bini, L.. - In: JOURNAL OF PROTEOMICS. - ISSN 1874-3919. - Jul 21;72:(2009), pp. 853-864.
Solubilization methods and reference 2-DE map of cow milk fat globules.
GREPPI, Gianfranco;
2009-01-01
Abstract
Milk fat globules (MFGs) are secretory vesicles assembled and secreted by mammary epithelial cells during lactation. They consist of fat globules surrounded by a lipid bilayer membrane which is derived from the apical membrane of the lactating cells. MFGs contain, besides lipids, proteins from the apical plasma membrane and from the cytoplasmatic material. Their peculiar vesicle nature makes them a suitable and easily available source of biological material in monitoring the physiopathological state of the mammary gland. Unfortunately, the conspicuous lipidic component of MFGs consistently limits protein extraction and purification for MFG proteomic investigations. This work deals with the development of a suitable procedure forI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
