sem low vacuum study of fat transfer coleman’s technique: effect of centrifugation and sedimentation on adipocyte morphology

The concept of beauty has always been important for man, if we think that cosmetics existed in ancient times and the body care had a crucial role. Among various techniques to repair physical defects exist lipofi lling, that is an explant of fat tissue from a part of body and the implant in other areas; however, autologous fat graft is not very simple and often it causes damages to the cells. The procedure used is Coleman’s technique and, in literature, we fi nd many variations of this method to overcome its limitations such as reabsorption of the implanted cells, formation of oil cysts and fi brous tissue. Today, there are some works that study what kind of modifi cations we can fi nd utilizing Coleman’s technique but no one of these analyses with SEM the alterations that may suff er the fat. Therefore, our work is to evaluate changes induced by this procedure and to validate SEM for analyses of the tissue explanted