Red deer(Cervus elaphus) haveapronouncedseasonalityintheirphysiology.Theaimof this studywastoassesstheeffectoftheseasononreddeerresponsivenesstoskintesting with thephytohaemagglutinin(PHA)mitogen.Studysubjectsincluded270farmedadult red deer(19stagsand251hinds).TheskintestingwascarriedoutbetweenJanuary2009 and August2010.Theanimalswereinjectedintradermallywitha0.1mlvolumecontaining 250g ofPHAdilutedinphosphatebufferedsaline.Theskinfoldthicknesswasmeasured immediately priortoinjectionand72hafteradministration,alwaysbythesamepersonand with threerepeatspermeasurement.Singleeffectsofsexandtimeonskintestresponsive- ness weresignificant(p < 0.001)aswellastheirinteraction(p < 0.001).Inwinter(January), and consideringtheaverageoftwoyears,theskinfoldincreaseinresponsetotheintra- dermal injectionof250g PHAwas2.1timeslargerinstagsand1.4timesinhindsthan in summer(August).Whilestagshad1.3timeslargerresponsesthanhindsinwinter,the inverse occurredinsummer,with1.1timeslargerresponsesinhinds.Wealsoevidenced a limitedinter-annualvariationofskinfoldincreaseinresponsetoPHAinreddeer.These findings haveimportantconsequencesregardingtheinterpretationofskintestresultsin the ante-mortemdiagnosisoftuberculosisandparatuberculosis,atleastindeer.
The testing season affects red deer skinfold increase in response to phytohaemagglutinin / Fernández de Mera*, Ig; Jaroso, R; Martín Hernando, Mp; Queiros, J; Carta, Tania; Ortiz, Ja; Vicente, J; Gortázar, C.. - In: PREVENTIVE VETERINARY MEDICINE. - ISSN 0167-5877. - 100:(2011), pp. 79-83. [10.1016/j.prevetmed.2011.02.011]
The testing season affects red deer skinfold increase in response to phytohaemagglutinin
CARTA, Tania;
2011-01-01
Abstract
Red deer(Cervus elaphus) haveapronouncedseasonalityintheirphysiology.Theaimof this studywastoassesstheeffectoftheseasononreddeerresponsivenesstoskintesting with thephytohaemagglutinin(PHA)mitogen.Studysubjectsincluded270farmedadult red deer(19stagsand251hinds).TheskintestingwascarriedoutbetweenJanuary2009 and August2010.Theanimalswereinjectedintradermallywitha0.1mlvolumecontaining 250g ofPHAdilutedinphosphatebufferedsaline.Theskinfoldthicknesswasmeasured immediately priortoinjectionand72hafteradministration,alwaysbythesamepersonand with threerepeatspermeasurement.Singleeffectsofsexandtimeonskintestresponsive- ness weresignificant(p < 0.001)aswellastheirinteraction(p < 0.001).Inwinter(January), and consideringtheaverageoftwoyears,theskinfoldincreaseinresponsetotheintra- dermal injectionof250g PHAwas2.1timeslargerinstagsand1.4timesinhindsthan in summer(August).Whilestagshad1.3timeslargerresponsesthanhindsinwinter,the inverse occurredinsummer,with1.1timeslargerresponsesinhinds.Wealsoevidenced a limitedinter-annualvariationofskinfoldincreaseinresponsetoPHAinreddeer.These findings haveimportantconsequencesregardingtheinterpretationofskintestresultsin the ante-mortemdiagnosisoftuberculosisandparatuberculosis,atleastindeer.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
