Thirty-two S. aureus strains isolated from sheep subclinical mastitis (SM) were analysed by multiplex PCR for staphylococcal enterotoxins (SEs) and virulence factor encoding genes. All strains were tested for SEs genes (sea-e, seh, sek, sel, sem, seo) and TSST-1 (tst). SEA-D production was tested using RPLA. All strains were also analysed for the following genes: gyrA (gyrase), agr I-IV alleles (accessory gene regulator), virulence factors eta, etb, etd (exfoliatins), lukF-PV and lukS-PV (Panton-Valentine leukocidin), lukE and lukD (LukE-LukD leukocidin), lukM (LukM leukocidin), hlg (hemolysin γ), hlgv (hemolysin γ variant) and hlb (hemolysin β), edin (epidermal cell differentiation inhibitor), mecA (methicillin resistance determinant). In eight out of thirty-two strains sea, sel and tst were detected, while three isolates harboured the seh. Genes sea, seb, sed, see, sek, sem and seo were not found. RPLA also detected SEC in ten strains. RPLA and PCR results in four cases did not agree. Further test conducted on these strains by ELISA given concordances with PCR. agr, hlgv and hlb were found in all strains. Twenty-three isolates harboured edin, nineteen luk-E and luk-D, and eight lukM. mecA, eta, etb, etd, hlg, lukF-PV and lukS-PV were not detected. S. aureus enterotoxigenic strains are less frequently found in sheep with SM than as has been reported for sheep with clinical mastitis. Six different profiles were observed based on the association of SEs and virulence genes.
Detection of enterotoxins and virulence genes in Staphylococcus aureus strains isolated from sheep subclinical mastitis / DE SANTIS, Enrico Pietro Luigi; Mureddu, A; Mazzette, Rina; Scarano, C; Bes, M.. - (2005), pp. 504-510. (Intervento presentato al convegno 4thIDF International Mastitis Conference) [10.3920/978-90-8686-550-5].
Detection of enterotoxins and virulence genes in Staphylococcus aureus strains isolated from sheep subclinical mastitis
DE SANTIS, Enrico Pietro Luigi;MAZZETTE, Rina;Scarano C;
2005-01-01
Abstract
Thirty-two S. aureus strains isolated from sheep subclinical mastitis (SM) were analysed by multiplex PCR for staphylococcal enterotoxins (SEs) and virulence factor encoding genes. All strains were tested for SEs genes (sea-e, seh, sek, sel, sem, seo) and TSST-1 (tst). SEA-D production was tested using RPLA. All strains were also analysed for the following genes: gyrA (gyrase), agr I-IV alleles (accessory gene regulator), virulence factors eta, etb, etd (exfoliatins), lukF-PV and lukS-PV (Panton-Valentine leukocidin), lukE and lukD (LukE-LukD leukocidin), lukM (LukM leukocidin), hlg (hemolysin γ), hlgv (hemolysin γ variant) and hlb (hemolysin β), edin (epidermal cell differentiation inhibitor), mecA (methicillin resistance determinant). In eight out of thirty-two strains sea, sel and tst were detected, while three isolates harboured the seh. Genes sea, seb, sed, see, sek, sem and seo were not found. RPLA also detected SEC in ten strains. RPLA and PCR results in four cases did not agree. Further test conducted on these strains by ELISA given concordances with PCR. agr, hlgv and hlb were found in all strains. Twenty-three isolates harboured edin, nineteen luk-E and luk-D, and eight lukM. mecA, eta, etb, etd, hlg, lukF-PV and lukS-PV were not detected. S. aureus enterotoxigenic strains are less frequently found in sheep with SM than as has been reported for sheep with clinical mastitis. Six different profiles were observed based on the association of SEs and virulence genes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
