X-adrenoleukodystrophy (X-ALD) is an inherited neurodegenerative disorder characterized by impaired peroxisomal beta-oxidation. It has been shown that conjugated linoleic acid (CLA) intake in humans increased plasma levels of isoprostanes . This increase has been taken as an evidence of oxidative stress triggered by CLA. This compound and 8-iso-PGF2alpha ( IP), have peroxisomal bet-oxidation. The aim of our study was to investigate the metabolism of CLA and its influence on IP formation, in vivo and in fibroblasts from X-ALD. We challenged rats fed CLA with a single dose of CCl4. The results showed that IP and its precursor arachidonic acid hydroperoxides increased significantly in the liver of rats challenged with CCl4. On the other hand, the peroxisomal beta oxidation products of IP, diinor (DIN), was found only in the free form and significantly lower in the CLA group with respect to the control groups. To further investigate whether there is a competition between CLA and IP at cellular level, we incubated human fibroblasts from healthy subjects or patients affected by X-ALD with CLA and/or commercially available IP. In X-ALD fibroblasts the formation of DIN was significantly lower, in both normal and ALD cells, the presence of CLA significantly inhibited the formation of DIN from IP.
Metabolism of 8-iso-PGF2alpha and conjugated linoleic acid (CLA) in vivo and in X-Adrenoleukodystrophy fibroblasts / Petroni, A; Iannone, Ca; Cordeddu, L; Murru, E; Carta, G; Melis, Mp; Bergamini, S; Blasevich, M; Carissimi, M; Marianne, O’Shea; DE SANTIS, Enrico Pietro Luigi; Banni, S.. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 21:(2007). (Intervento presentato al convegno II International Congress on CLA from Experimental Models to Human Application tenutosi a Villasimius (CA) nel 19-22 settembre 2007).
Metabolism of 8-iso-PGF2alpha and conjugated linoleic acid (CLA) in vivo and in X-Adrenoleukodystrophy fibroblasts
DE SANTIS, Enrico Pietro Luigi;
2007-01-01
Abstract
X-adrenoleukodystrophy (X-ALD) is an inherited neurodegenerative disorder characterized by impaired peroxisomal beta-oxidation. It has been shown that conjugated linoleic acid (CLA) intake in humans increased plasma levels of isoprostanes . This increase has been taken as an evidence of oxidative stress triggered by CLA. This compound and 8-iso-PGF2alpha ( IP), have peroxisomal bet-oxidation. The aim of our study was to investigate the metabolism of CLA and its influence on IP formation, in vivo and in fibroblasts from X-ALD. We challenged rats fed CLA with a single dose of CCl4. The results showed that IP and its precursor arachidonic acid hydroperoxides increased significantly in the liver of rats challenged with CCl4. On the other hand, the peroxisomal beta oxidation products of IP, diinor (DIN), was found only in the free form and significantly lower in the CLA group with respect to the control groups. To further investigate whether there is a competition between CLA and IP at cellular level, we incubated human fibroblasts from healthy subjects or patients affected by X-ALD with CLA and/or commercially available IP. In X-ALD fibroblasts the formation of DIN was significantly lower, in both normal and ALD cells, the presence of CLA significantly inhibited the formation of DIN from IP.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
