Purpose: Monocytes are effector cells of the non-specific immune response involved in the first recognition and response to pathogens. Sex hormones may play an important role in regulating the immune responses and monocytes possess functional sex hormone receptors. Therefore, the aim of this study was to evaluate whether the use of oral contraceptives (OCs) can affect monocyte activity. To this end, we evaluate the effect of OCs on pro-inflammatory and anti-inflammatory cytokines release by human monocytes in response to known agonists of TLR4 and TLR2 receptors. Methods: Primary human peripheral blood monocytes from healthy adult women treated (FOCs) or non-treated (Fs) with OCs were isolated during the follicular phase of their menstrual cycle and stimulated with either LPS or Pam2CSK4, in vitro. Release of TNF-α, IL-6, IL-10 and IL-8 was investigated at specific hours post-stimulation, by Enzyme Linked Immuno Sorbent Assay (ELISA). Results: Our data showed that both agonists used induced the release of TNF-α, IL-6 and IL-8 by Fs and FCOs monocytes, whereas IL-10 was only induced by LPS in both monocytes population, at 18 h. Differences in the kinetics of LPS-induced TNF-α were observed in FOCs monocytes, in comparison with Fs, but not quite significant. Of interest, a highly significant reduction of IL-8 detection, both in the basal condition and after the two stimulations, and of IL-10, after stimulation with LPS only, was observed in monocytes FCOs in comparison with Fs monocytes. Discussion: Our results indicate that the FCOs monocyte response to LPS and Pam2CSK4 is delayed (TNF-α and IL-10) or less intense (IL-8 and IL-10). These changes might reduce monocyte ability to activate neutrophils and endothelial cells, to recruit neutrophils and other granulocytes in the site of infection, and to suppress inflammation and macrophage activity. Conclusions: The use of COs might influence the immunomodulatory activity of monocytes during infectious processes. This work was supported by grant CPR.59632, LR72012MATTANA from the Regione Sardegna.
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
|Titolo:||Influence of oral contraceptives on monocytes activity (with implications on modulation of the immune response)|
|Data di pubblicazione:||2016|
|Appare nelle tipologie:||4.2 Abstract in Atti di convegno|