The aim of this work was the detection of MAP in human with CD and in ovine with natural and experimental PTBC, by in “situ” techniques. ISH showed strong signals in the macrophages infiltrating the mucosal and submucosal layers in PTBC samples and positive signals in macrophages of CD cases. Furthermore, ISPCR signals were more numerous than those obtained by ISH. This result indicate that ISPCR is able to detect also less copies of MAP DNA.
Detection ofMycobacterium aviumsubsp.paratuberculosis(MAP) in animals and human tissue / Rocca, Stefano; Leoni, Antonio; Sechi, Leonardo Antonio; Farigu, Serafina; Lissia, Amelia; Mingioni, Vindice; Pirino, Salvatore; Tanda, Francesco; Sanna, Maria Paola. - In: ATTI ... CONVEGNO NAZIONALE AIPVET. - ISSN 1825-2265. - (2005), pp. 19-22. (Intervento presentato al convegno 2. Congresso nazionale A.I.P.Vet.: atti).
Detection ofMycobacterium aviumsubsp.paratuberculosis(MAP) in animals and human tissue
Rocca, Stefano;Leoni, Antonio;Sechi, Leonardo Antonio;Farigu, Serafina;Lissia, Amelia;Mingioni, Vindice;Pirino, Salvatore;Tanda, Francesco;Sanna, Maria Paola
2005-01-01
Abstract
The aim of this work was the detection of MAP in human with CD and in ovine with natural and experimental PTBC, by in “situ” techniques. ISH showed strong signals in the macrophages infiltrating the mucosal and submucosal layers in PTBC samples and positive signals in macrophages of CD cases. Furthermore, ISPCR signals were more numerous than those obtained by ISH. This result indicate that ISPCR is able to detect also less copies of MAP DNA.| File | Dimensione | Formato | |
|---|---|---|---|
|
Sanna_MP_ContrCongresso_2005_Detection.pdf
accesso aperto
Tipologia:
Versione editoriale (versione finale pubblicata)
Licenza:
Non specificato
Dimensione
6.12 MB
Formato
Adobe PDF
|
6.12 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
