The bacterial genusHelicobactercontains at least 18 species which colonise the gastrointestinal tract of several mammalian and avian hosts. In literature,Helicobacterspp. infection in cats is reported with a prevalence between 41% and 100%. Several studies showed the presence in cat ofH. pylorieH. heilmannii, potential zoonotic agents. The aim of this work was to show the presence ofHelicobacterin feline gastric washings collected in small animals veterinary clinics. The samples were collected from cats presented for surgical operations. Eleven gastric fluids were centrifuged and submitted to cytological examination (May Grünwald-Giemsa). Besides, the DNA extracted from samples was examined by PCR, in order to reveal a fragment of 16S rRNA gene ofHelicobacter. All tested samples resulted positive. Nucleotide sequencing allowed to identifyH. heilmanniiin one case. The collection method revealed to be useful in monitoringHelicobacterspread in feline population, being non-invasive, reliable and easily feasible.
Identification ofHelicobacterspp. in gastric washings in cat / Appino, Simonetta; Pregel, Paola; Gastaldi, Giovanna; Palmierini, Domenico. - In: ATTI ... CONVEGNO NAZIONALE AIPVET. - ISSN 1825-2265. - (2005), p. 125. (Intervento presentato al convegno 2. Congresso nazionale A.I.P.Vet.: atti).
Identification ofHelicobacterspp. in gastric washings in cat
Appino, Simonetta;
2005-01-01
Abstract
The bacterial genusHelicobactercontains at least 18 species which colonise the gastrointestinal tract of several mammalian and avian hosts. In literature,Helicobacterspp. infection in cats is reported with a prevalence between 41% and 100%. Several studies showed the presence in cat ofH. pylorieH. heilmannii, potential zoonotic agents. The aim of this work was to show the presence ofHelicobacterin feline gastric washings collected in small animals veterinary clinics. The samples were collected from cats presented for surgical operations. Eleven gastric fluids were centrifuged and submitted to cytological examination (May Grünwald-Giemsa). Besides, the DNA extracted from samples was examined by PCR, in order to reveal a fragment of 16S rRNA gene ofHelicobacter. All tested samples resulted positive. Nucleotide sequencing allowed to identifyH. heilmanniiin one case. The collection method revealed to be useful in monitoringHelicobacterspread in feline population, being non-invasive, reliable and easily feasible.| File | Dimensione | Formato | |
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