Nickel has been shown to be an essential trace element involved in the metabolism of several species of bacteria, archea, plant and may yet be found to play a role in the metabolism of higher organisms. However, the carcinogenicity of certain nickel compounds has been confirmed by the combination of epidemiological evidence in humans and carcinogenesis bioassays in animals. The molecular mechanisms of nickel-induced carcinogenesis include interactions of this metal with major chromatin components causing alterations in gene expression rather than by direct and mammalian cells. It has preference to specific lysine residues in the N-terminal tail of histone H4, in which the sites of acetylation are clustered.Here we present our recent results on the coordination ability of Ni(II) to the N-terminal tail of Histone H4 using NMR spectroscopy. A series of 1D, 2D Tocsy and Noesy1H NMR spectra of the tail with increasing nickel concentration to the final molar ratio 1:1, were acquired.

NMR study of Nickel binding to N-tail of histone H4 / Zoroddu, Maria Antonietta; Peana, Massimiliano Francesco; Medici, Serenella. - (2005). (Intervento presentato al convegno 8. FIGIPAS meeting in inorganic chemistry).

NMR study of Nickel binding to N-tail of histone H4

Zoroddu, Maria Antonietta;Peana, Massimiliano Francesco;Medici, Serenella
2005-01-01

Abstract

Nickel has been shown to be an essential trace element involved in the metabolism of several species of bacteria, archea, plant and may yet be found to play a role in the metabolism of higher organisms. However, the carcinogenicity of certain nickel compounds has been confirmed by the combination of epidemiological evidence in humans and carcinogenesis bioassays in animals. The molecular mechanisms of nickel-induced carcinogenesis include interactions of this metal with major chromatin components causing alterations in gene expression rather than by direct and mammalian cells. It has preference to specific lysine residues in the N-terminal tail of histone H4, in which the sites of acetylation are clustered.Here we present our recent results on the coordination ability of Ni(II) to the N-terminal tail of Histone H4 using NMR spectroscopy. A series of 1D, 2D Tocsy and Noesy1H NMR spectra of the tail with increasing nickel concentration to the final molar ratio 1:1, were acquired.
2005
NMR study of Nickel binding to N-tail of histone H4 / Zoroddu, Maria Antonietta; Peana, Massimiliano Francesco; Medici, Serenella. - (2005). (Intervento presentato al convegno 8. FIGIPAS meeting in inorganic chemistry).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/262637
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