Studio epidemiologico sul rapporto tra infezione daPapillomaviruse carcinoma della cervice uterina nella popolazione del Nord Sardegna. Individuazione di nuovi marker indicatori dell’attività replicativa ed oncogena dei virus

Aim: Within the research project about the epidemiology of infection by papillomavirus (HPV), North Sardinia, we conducted a retrospective study in order to define the prevalence of infection, the genotypes with greater spread over vast areas and to describe some determinants of virulence. Methods: were analyzed No 155 paraffin-embedded biopsy specimens taken from patients with cervical lesions classified as mild (CIN1), moderate (CIN2) and severe dysplasia (CIN3) and invasive carcinoma. Biological samples, after the histological classification performed at the Institute of Pathology, were sent to the Institute of Hygiene and MP for the research of viral DNA with commercial assay (INNO-LiPA). Results: The results showed that 64.5% of the samples tested positive for HPV DNA research, in particular, the HPV16 genotype was found with a frequency of 67%, followed dall'HPV51 (24%), HPV52 ( 9%), HPV45 (6%), HPV 33 (5%) and HPV18 (4%). To report a 31% sustainable packaging, 18%, HPV 16 and 51. Conclusion: the results revealed a prevalence of less than expected whereas 80,6% of biopsy specimens were from patients with degree of dysplasia, moderate / severe, as well as invasive carcinoma. The survey also revealed the presence of high-risk genotypes less common than in other areas, such as HPV51, something that could be an epidemiological peculiarity of our region.