8 Tesi di dottorato

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8.1 Tesi di dottorato 324
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Bioinspired by Nature, Designed for Flow: Engineering Functional Interfaces for Small-Caliber Vascular Grafts

This thesis investigates the use of marine sulfated polysaccharides (MSPs) and other natural bioactive molecules for the design of next-generation small-caliber vascular grafts. Specifically: Chapter 2 provides a comprehensive review of MSPs as biofunctional agents for enhancing hemocompatibility and endothelialization in tissue-engineered vascular grafts. MSPs, including fucoidans, carrageenans, and fucosylated chondroitin sulfates (FCS), have attracted increasing attention due to their stru...

Artificial Intelligence Standardisation for AI Regulation. A study on the role and limitations of harmonised standards in the context of the European Union legislation on AI

This dissertation examines the role of technical standards in the implementation of the EU AI Act and questions a central assumption behind the Act’s regulatory design, i.e., that harmonised standards can translate high-level legal requirements for trustworthy AI into concrete technical specifications. Rather than treating standardisation mainly as a question of institutional legitimacy, the thesis asks whether standards can perform the regulatory work assigned to them. Its main contribution ...

Prevalence, molecular characterization, and risk factors of canine Giardiasis in Lebanon, Middle East and Sardinia, Italy

The present dissertation investigates the epidemiology, molecular characteristics, and risk factors of canine giardiasis in Sardinia (Italy) and Lebanon. Giardia duodenalis is a globally important intestinal protozoan with zoonotic potential, yet data from these regions are limited. This study combines copromicroscopy and molecular analysis to evaluate dogs as reservoirs within a One Health framework. Faecal samples were analyzed using zinc sulphate flotation and light microscopy (centrifugal...

ADVANCING IN VITRO EMBRYO PRODUCTION IN PREPUBERTAL SMALL RUMINANTS: OPTIMIZING CULTURE SYSTEMS TO ACCELERATE GENETIC GAIN AND ENHANCE ENVIRONMENTAL SUSTAINABILITY

In vitro embryo production (IVEP) from prepubertal donors represents a promising strategy to accelerate genetic gain in livestock; however, the reduced developmental competence of prepubertal oocytes remains a major limitation. This is mainly due to intrinsic oocyte immaturity and suboptimal in vitro maturation (IVM) conditions. Therefore, improving culture systems and identifying reliable biomarkers of oocyte quality are crucial to enhance the efficiency of juvenile IVEP programs. Three-dime...

Standardization of 3D Spheroid Generation and Mass Density Profiling: Development of a Biofluidic Instrument and Application to Ovine Trophoblast Models

L'obiettivo generale di questo progetto di dottorato è stabilire una pipeline tecnologica e biologica completa e coerente per la generazione, la preservazione e la caratterizzazione biofisica di modelli cellulari tridimensionali (3D) di interesse per le scienze veterinarie. Questo lavoro si muove dal design dei materiali all'applicazione biologica, integrando scaffold microstrutturati a base di agarosio, strategie di conservazione a breve termine e innovative tecnologie microfluidiche per cre...

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