Sviluppo di sistemi nanoparticellari contenenti idrossipropil-β-ciclodestrina per il rilascio colonspecifico di farmaci

Diclofenac Sodium (DS) was chosen as model drug for colonic drug delivery because of its large use against the Arthritis rheumatoid therapy, that determines an increment of pain during the early morning. This aspect drove this study to the preparation of colon specific formulations able to guarantee the absorption of the drug several hours after the oral administration.Nanoparticles hydroxypropyl-β-cyclodextrin (HP) based containing DS were prepared. The efficacy of HP as gastroresistant polymer was evaluated. Comparison between gastroresistant properties of HP and the characteristics of gastroresistant polymer Eudragit L100®was done. Moreover synergic effects obtained by the mix of those two excipients were evaluated.The second part of the work was based on the study of HP capability to favorite colon specific delivery of DS from Solid Lipid Nanoparticles. Two kinds of SLN loaded with DS were produced: a series of formulations containing both HP and lipid, a second series composed exclusively by the lipid.The third chapter is based on the development of a new method of entrapment of proteic antigens on polymeric nanoparticles. The innovation of the study was due to the method of protein loading. Aim of this work was to adsorb proteins on the surface of preformed nanoparticles. Several parameters were studied in order to evaluate the best way to obtain the maximum adsorption of the protein.