This work analyses the diversity of rhizobia associated to nodules of different leguminous shrubs in Sardinia, to date almost unknown. Seventy-eight rhizobial strains were isolated from root nodules ofAstragalus terracianoi,Cytisus villosus,Coronilla valentinaandGenista sardoa.This collection was characterized by genomic and phenotypic features. Phylogenies were inferred from their 16S and three symbiotic genes (nodA,nodCandnifH). The genetic analysis revealed that the rhizobia isolated fromCytisus villosus. nodules belong to generaBradyrhizobium, and that the rhizobia isolated fromAstragalus terracianoiandCoronilla valentinanodules belong to generaMesorhizobium, and that the rhizobia isolated fromGenista sardoanodules belong to generaRhizobium,PhyllobacteriumandBradyrhizobium. The Microplate GenIII BiologTM has allowed a metabolic fingerprinting of isolates by using 71 carbon sources and 23 Chemical Sensitivity Assays.The symbiotic tests showed differences among all rhizobia isolates with respect to their effectiveness and infectivity; for example, as withGenista sardoa, that had been infected from a single strain or as withCytisus villosus, that although had been nodulated by all inoculated strains, did not produce dry weights different from the control plants. Moreover, it has been identified to be the best interaction microsymbiont-host plant that could be utilized in a revegetation project.like (as) potential strategy in revegetation project.
Caratterizzazione fenotipica e molecolare di batteri azotofissatori simbionti associati a leguminose arbustive della Sardegna(2012 Jan 20).
Caratterizzazione fenotipica e molecolare di batteri azotofissatori simbionti associati a leguminose arbustive della Sardegna
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2012-01-20
Abstract
This work analyses the diversity of rhizobia associated to nodules of different leguminous shrubs in Sardinia, to date almost unknown. Seventy-eight rhizobial strains were isolated from root nodules ofAstragalus terracianoi,Cytisus villosus,Coronilla valentinaandGenista sardoa.This collection was characterized by genomic and phenotypic features. Phylogenies were inferred from their 16S and three symbiotic genes (nodA,nodCandnifH). The genetic analysis revealed that the rhizobia isolated fromCytisus villosus. nodules belong to generaBradyrhizobium, and that the rhizobia isolated fromAstragalus terracianoiandCoronilla valentinanodules belong to generaMesorhizobium, and that the rhizobia isolated fromGenista sardoanodules belong to generaRhizobium,PhyllobacteriumandBradyrhizobium. The Microplate GenIII BiologTM has allowed a metabolic fingerprinting of isolates by using 71 carbon sources and 23 Chemical Sensitivity Assays.The symbiotic tests showed differences among all rhizobia isolates with respect to their effectiveness and infectivity; for example, as withGenista sardoa, that had been infected from a single strain or as withCytisus villosus, that although had been nodulated by all inoculated strains, did not produce dry weights different from the control plants. Moreover, it has been identified to be the best interaction microsymbiont-host plant that could be utilized in a revegetation project.like (as) potential strategy in revegetation project.File | Dimensione | Formato | |
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