Influenza dell'azione degli antiossidanti nella capacità fecondante dei gameti maschili e femminili

A significant reduction in spermatozoa’s and ovocites’s level antioxidants has been reported as cause of susceptibility of these cells to peroxidative injuries after cryopreservation andin vitromaturation (IVM). The aim of this study was to investigate whether there are qualitative differences of antioxidant capacity content in follicular fluids collected from ovarian follicles in seminal plasma and sperm cell extract among boar and ram at different times of the year. Samples were processed rapidly and kept frozen at − 80 °C until assayed. TEAC, SOD, MDA and ROMs of PFF, seminal plasma and sperm cell extract was determined using assays as described in literature and modified. Obtained results showed statistically significant differences for all PFF’s assays, while for boar semen’s d-Roms test, MDA and seminal plasma’s SOD wasn’t s statistically significant differences. In ram’s semen, only Roms’ assay wasn’t statistically significant.Our results suggest that that the quality of the follicular fluid (and the ability to increase the IVM) as well as quality of boar and ram semen, may be linked to their oxidative state. This finding can be applied to develop protocol to optimize IVM in pig and optimize semen cryopreservation protocols, but may also have an application in developing therapeutic protocols for male infertility related to a decrease in sperm antioxidant defense system.