Study of the molecular patterning in stem cells exposed to biophysical stimuli: a new vision of regenerative medicine

Regenerative medicine is a novel applied field of medical management based on the application of stem cell technology and tissue engineering in order to replace or regenerate human tissues and organs and restore their functions. The regenerative medicine has the prospective to help scientists and clinicians while planning early-intervention treatments for traumatic injury or degenerative diseases, by regrowth or replacement of cells or tissues.However, the challenges in this field are still countless, as (1) which type of cells are suitable (2) which is the best protocol to commit all the stem cells toward a specific phenotype with the 100% of yield, (3) drop cell manipulation.At the same time, physical energy were found capable to interact with cell physiology and increase their performance. In the clinical practice physical therapies enhance the quality and time of spontaneous tissues regeneration.Here, I focused on the effect of physical stimuli by Radio electric asymmetric conveyor (REAC) and Extra corporeal shock wave (SW) and their interaction with cells or stem cell behaviour.In particular, during the modulation of the main molecular patterning controlling cellular senescence, differentiation toward specific phenotypes and proliferation, in order to figure out the targets of action, to enhance their performance for future therapeutic applications.