Deinococcus Radiodurans (Dr) is a bacterium which has drawn a lot of attention on its extraordinary radioprotective properties since its discovery in 1956. Different authors have reported that Mn-peptide complexes, isolated from Dr cell-free extract, could act as scavenging agents by neutralizing ROS molecules induced through radiation exposure [1]. In this study we have investigated the interaction between Mn(II) and two different peptides, DP1(DEHGTAVMLK) and DP2 (THMVLAKGED), as a model of the antioxidative mechanism in Dr. The amino acid composition of these peptides was chosen randomly on the basis of their natural abundance in the Dr protein-free cell extract (ultrafiltrate). We have focused our attention on the coordination of Mn(II) with DP1 and DP2 peptides by using NMR, EPR and ESI-MS techniques. Additionally, competition experiments have been done to study the equilibrium between Mn-peptides and Mnphosphate complexes, which, according to recent findings, could be a key point to explain the radiation resistance of Dr [2].

Mn-peptides complexes involved in γ-radiation resistance in Deinococcus radiodurans / Giancarlo, Simula; Massimiliano, Peana; Serenella, Medici; Costantino, Solinas; Maria, Antonietta Zoroddu. - (2016), pp. 9-9. ((Intervento presentato al convegno XIV edizione del convegno La Parola ai Giovani 2016 tenutosi a Complesso Didattico – Via Vienna 2 – Sassari nel 28 Ottobre 2016.

Mn-peptides complexes involved in γ-radiation resistance in Deinococcus radiodurans

PEANA, Massimiliano Francesco;MEDICI, Serenella;SOLINAS, Costantino;ZORODDU, Maria Antonietta
2016

Abstract

Deinococcus Radiodurans (Dr) is a bacterium which has drawn a lot of attention on its extraordinary radioprotective properties since its discovery in 1956. Different authors have reported that Mn-peptide complexes, isolated from Dr cell-free extract, could act as scavenging agents by neutralizing ROS molecules induced through radiation exposure [1]. In this study we have investigated the interaction between Mn(II) and two different peptides, DP1(DEHGTAVMLK) and DP2 (THMVLAKGED), as a model of the antioxidative mechanism in Dr. The amino acid composition of these peptides was chosen randomly on the basis of their natural abundance in the Dr protein-free cell extract (ultrafiltrate). We have focused our attention on the coordination of Mn(II) with DP1 and DP2 peptides by using NMR, EPR and ESI-MS techniques. Additionally, competition experiments have been done to study the equilibrium between Mn-peptides and Mnphosphate complexes, which, according to recent findings, could be a key point to explain the radiation resistance of Dr [2].
Mn-peptides complexes involved in γ-radiation resistance in Deinococcus radiodurans / Giancarlo, Simula; Massimiliano, Peana; Serenella, Medici; Costantino, Solinas; Maria, Antonietta Zoroddu. - (2016), pp. 9-9. ((Intervento presentato al convegno XIV edizione del convegno La Parola ai Giovani 2016 tenutosi a Complesso Didattico – Via Vienna 2 – Sassari nel 28 Ottobre 2016.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/169312
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