Brain microdialysis is an analytical technique used for the dynamic monitoring of brain neurochemistry in awake, freely moving animals. This technique requires the insertion of a small dialysis catheter, called a microdialysis probe, into a specific brain region, and its perfusion with an artificial extracellular fluid. The microdialysate samples, obtained from the probe outlet, can be analysed using high-performance liquid chromatography with electrochemical detection for the quantification of oxidizable molecules recovered from the extracellular space. In this chapter, we describe a protocol for performing a microdialysis setup and experiment in freely moving rats and mice. Furthermore, the high-performance liquid chromatographic determination of ascorbic acid, uric acid, catecholamines, indolamines and derivatives is described in detail.
Brain microdialysis in freely moving animals / Bazzu, Gianfranco; Biosa, A; Farina, Donatella; Spissu, Y; Calia, G; Dedola, S; Rocchitta, Gaia Giovanna Maria; Migheli, Rossana; Serra, Pier Andrea; Desole, Maria Speranza. - 846:(2012), pp. 365-381. [10.1007/978-1-61779-536-7_31]
Brain microdialysis in freely moving animals.
BAZZU, Gianfranco;FARINA, Donatella;ROCCHITTA, Gaia Giovanna Maria;MIGHELI, Rossana;SERRA, Pier Andrea;DESOLE, Maria Speranza
2012-01-01
Abstract
Brain microdialysis is an analytical technique used for the dynamic monitoring of brain neurochemistry in awake, freely moving animals. This technique requires the insertion of a small dialysis catheter, called a microdialysis probe, into a specific brain region, and its perfusion with an artificial extracellular fluid. The microdialysate samples, obtained from the probe outlet, can be analysed using high-performance liquid chromatography with electrochemical detection for the quantification of oxidizable molecules recovered from the extracellular space. In this chapter, we describe a protocol for performing a microdialysis setup and experiment in freely moving rats and mice. Furthermore, the high-performance liquid chromatographic determination of ascorbic acid, uric acid, catecholamines, indolamines and derivatives is described in detail.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.