Neural and glial cells are continuously exposed in vivo to oxidative stress through by-products of oxygen metabolism, i.e. ROS, with differential susceptibility to certain pathological conditions. Oxidative and nitrosative stresses are seen during hyperglycaemia when the excess of glucose resulted in an increase in ROS with subsequent cellular lesions, such as impairment of cytoskeletal network. A number of evidences support the idea that sexual hormones can exert positive effects on oxidative-stress injuries. In this study the hypothesis has been evaluated that high levels of D-glucose can induce deleterious effects on cytoskeletal proteins in mouse neuroblastoma (C1300) and rat glioma (C6) undifferentiated cell lines. Moreover, the possible neuroprotective action of testosterone (T) in cells exposed to high-glucose concentration was tested.Results revealed alterations in cell morphology, growth rate, viability and catalase activity in D-glucose cells. In addition, an increase in 3-nitro-L-tyrosine and a downregulation of all tubulin isoforms examined was found with Western blot analysis and immunofluorescence. All these changes were more severe in C1300 than in C6 cells. In the presence of testosterone (D-glucose+T cells) all the parameters monitored in C1300 cells were very similar to those from control (C1300 panel, Figs.1-4), whereas testosterone was not able to recover significantly C6 cells (C6 panel, Figs. 5-8). In both the cell lines, neither showed actin nor GFAP any changes. Finally, Western blot analysis revealed a higher amount of androgen receptors in C1300 than in C6 cells. High-D-glucose exposure led to severe microtubular alterations and the hyperglycaemia-induced neuronal injuries could be prevented with the addition of testosterone to the medium. All these results were well-evident in the cells of neural origin. In contrast, glial cells were found to be more resistant to high-glucose oxidative stress, so that no noticeable testosterone protective effects could be seen. This different pattern between the two cell lines may be supported by the fact that testosterone effects are mediated by androgen receptors which are expressed to a larger extent in neural than in glial cells.

Oxidative-induced microtubular modifications and different testosterone neuroprotective effects on neuroblastoma and glioma D-Glucose-exposed cells / Gadau, Sergio Domenico; Zedda, Marco; Lepore, Gianluca; Arru, B; Mura, A; Farina, Vittorio. - (2008), pp. 82-82. ((Intervento presentato al convegno 27th EAVA Congress tenutosi a Budapest nel 23-26/07/08.

Oxidative-induced microtubular modifications and different testosterone neuroprotective effects on neuroblastoma and glioma D-Glucose-exposed cells

GADAU, Sergio Domenico
;
ZEDDA, Marco;LEPORE, Gianluca;FARINA, Vittorio
2008-01-01

Abstract

Neural and glial cells are continuously exposed in vivo to oxidative stress through by-products of oxygen metabolism, i.e. ROS, with differential susceptibility to certain pathological conditions. Oxidative and nitrosative stresses are seen during hyperglycaemia when the excess of glucose resulted in an increase in ROS with subsequent cellular lesions, such as impairment of cytoskeletal network. A number of evidences support the idea that sexual hormones can exert positive effects on oxidative-stress injuries. In this study the hypothesis has been evaluated that high levels of D-glucose can induce deleterious effects on cytoskeletal proteins in mouse neuroblastoma (C1300) and rat glioma (C6) undifferentiated cell lines. Moreover, the possible neuroprotective action of testosterone (T) in cells exposed to high-glucose concentration was tested.Results revealed alterations in cell morphology, growth rate, viability and catalase activity in D-glucose cells. In addition, an increase in 3-nitro-L-tyrosine and a downregulation of all tubulin isoforms examined was found with Western blot analysis and immunofluorescence. All these changes were more severe in C1300 than in C6 cells. In the presence of testosterone (D-glucose+T cells) all the parameters monitored in C1300 cells were very similar to those from control (C1300 panel, Figs.1-4), whereas testosterone was not able to recover significantly C6 cells (C6 panel, Figs. 5-8). In both the cell lines, neither showed actin nor GFAP any changes. Finally, Western blot analysis revealed a higher amount of androgen receptors in C1300 than in C6 cells. High-D-glucose exposure led to severe microtubular alterations and the hyperglycaemia-induced neuronal injuries could be prevented with the addition of testosterone to the medium. All these results were well-evident in the cells of neural origin. In contrast, glial cells were found to be more resistant to high-glucose oxidative stress, so that no noticeable testosterone protective effects could be seen. This different pattern between the two cell lines may be supported by the fact that testosterone effects are mediated by androgen receptors which are expressed to a larger extent in neural than in glial cells.
Oxidative-induced microtubular modifications and different testosterone neuroprotective effects on neuroblastoma and glioma D-Glucose-exposed cells / Gadau, Sergio Domenico; Zedda, Marco; Lepore, Gianluca; Arru, B; Mura, A; Farina, Vittorio. - (2008), pp. 82-82. ((Intervento presentato al convegno 27th EAVA Congress tenutosi a Budapest nel 23-26/07/08.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/76165
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