Thirty S. aureus strains isolated from goat’s bulk milk were analysed by multiplex PCR for the following genes: gyrA (gyrase); agr I-IV alleles (accessory gene regulator); sea-e, seh, sek, sel, sem, seo (enterotoxins genes); tst (TSST-1); eta, etb, etd (exfoliatins); lukF-PV and lukS-PV (Panton-Valentine leukocidin); lukE and lukD (lukE-lukD leukocidin); lukM (lukM leukocidin); hlg, hlgv and hlb (γ, γ variant and ß hemolysines); edin (epidermal cell differentiation inhibitor); mecA (methicillin resistance determinant). SEA-E production was also tested using ELISA method. Genes agr III, sec, sel, tst, LukE-lukD, lukM, hlgv and hlb were found in majority of the strains, while agr IV, seb, seh, mecA, eta, etb, etd, lukF-PV and lukS-PV were not found. Ten different profiles on the bases of the association of agr alleles, SEs and virulence genes were observed.
DETECTION OF ENTEROTOXINS AND VIRULENCE GENES IN STAPHYLOCOCCUS AUREUS ISOLATED FROM GOAT’S BULK MILK / Scarano, Christian; S., Virdis; A. L., Pilo; P., Marongiu; DE SANTIS, Enrico Pietro Luigi; M., Bes. - In: BULLETIN - FÉDÉRATION INTERNATIONALE DE LAITERIE. - ISSN 0250-5118. - (2007). (Intervento presentato al convegno 5th International Symposium on the Challenge to Sheep and Goats Milk Sectors nel 18-20 april 2007).
DETECTION OF ENTEROTOXINS AND VIRULENCE GENES IN STAPHYLOCOCCUS AUREUS ISOLATED FROM GOAT’S BULK MILK
SCARANO, Christian;DE SANTIS, Enrico Pietro Luigi;
2007-01-01
Abstract
Thirty S. aureus strains isolated from goat’s bulk milk were analysed by multiplex PCR for the following genes: gyrA (gyrase); agr I-IV alleles (accessory gene regulator); sea-e, seh, sek, sel, sem, seo (enterotoxins genes); tst (TSST-1); eta, etb, etd (exfoliatins); lukF-PV and lukS-PV (Panton-Valentine leukocidin); lukE and lukD (lukE-lukD leukocidin); lukM (lukM leukocidin); hlg, hlgv and hlb (γ, γ variant and ß hemolysines); edin (epidermal cell differentiation inhibitor); mecA (methicillin resistance determinant). SEA-E production was also tested using ELISA method. Genes agr III, sec, sel, tst, LukE-lukD, lukM, hlgv and hlb were found in majority of the strains, while agr IV, seb, seh, mecA, eta, etb, etd, lukF-PV and lukS-PV were not found. Ten different profiles on the bases of the association of agr alleles, SEs and virulence genes were observed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
