Thirty S. aureus strains isolated from goat’s bulk milk were analysed by multiplex PCR for the following genes: gyrA (gyrase); agr I-IV alleles (accessory gene regulator); sea-e, seh, sek, sel, sem, seo (enterotoxins genes); tst (TSST-1); eta, etb, etd (exfoliatins); lukF-PV and lukS-PV (Panton-Valentine leukocidin); lukE and lukD (lukE-lukD leukocidin); lukM (lukM leukocidin); hlg, hlgv and hlb (γ, γ variant and ß hemolysines); edin (epidermal cell differentiation inhibitor); mecA (methicillin resistance determinant). All strains were also analysed by PFGE and MLST. Genes agr III, sec, sel, tst, LukE-lukD, lukM, hlgv and hlb were found in majority strains, while agr IV, seb, seh, mecA, eta, etb, etd, lukF-PV and lukS-PV were not found. PFGE and MLST subdivided the strains respectively in 14 pulsotypes, 7 groups of similarity and 7 classes.
CARATTERIZZAZIONE GENOTIPICA E STUDIO DI CLONALITÀ DI CEPPI DI S. AUREUS ISOLATI DA LATTE DI CAPRA ALLA PRODUZIONE / Scarano, Christian; Corgiolu, G.; Virdis, S.; Marongiu, P.; DE SANTIS, Enrico Pietro Luigi; Bes, M.. - In: ATTI DELLA SOCIETÀ ITALIANA DELLE SCIENZE VETERINARIE. - ISSN 1825-4454. - (2006), pp. 451-452. (Intervento presentato al convegno LX Convegno Nazionale Società Italiana Delle Scienze Veterinarie tenutosi a Terrasini (PA) nel 27/30 Settembre 2006).
CARATTERIZZAZIONE GENOTIPICA E STUDIO DI CLONALITÀ DI CEPPI DI S. AUREUS ISOLATI DA LATTE DI CAPRA ALLA PRODUZIONE
SCARANO, Christian;DE SANTIS, Enrico Pietro Luigi;
2006-01-01
Abstract
Thirty S. aureus strains isolated from goat’s bulk milk were analysed by multiplex PCR for the following genes: gyrA (gyrase); agr I-IV alleles (accessory gene regulator); sea-e, seh, sek, sel, sem, seo (enterotoxins genes); tst (TSST-1); eta, etb, etd (exfoliatins); lukF-PV and lukS-PV (Panton-Valentine leukocidin); lukE and lukD (lukE-lukD leukocidin); lukM (lukM leukocidin); hlg, hlgv and hlb (γ, γ variant and ß hemolysines); edin (epidermal cell differentiation inhibitor); mecA (methicillin resistance determinant). All strains were also analysed by PFGE and MLST. Genes agr III, sec, sel, tst, LukE-lukD, lukM, hlgv and hlb were found in majority strains, while agr IV, seb, seh, mecA, eta, etb, etd, lukF-PV and lukS-PV were not found. PFGE and MLST subdivided the strains respectively in 14 pulsotypes, 7 groups of similarity and 7 classes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
