ORGANIZED BYDepartment of Animal Production, Bari, ItalyFaculty of Veterinary Medicine, Perugia, ItalyFaculty of Veterinary Medicine, Agricultural University, Tirana, Albania
A 3-steps equilibration method was compared to a single-step one in vitrification of cumulus-expanded (CE) and cumulus-compacted (CC) horse oocytes, both in GV and MII stage, obtained from ovaries of slaughtered mares. After thawing oocyte viability was assessed by nuclear maturation rate after in vitro culture for oocytes vitrified at the GV stage and by the presence of an intact zona pellucida and membrane after 2 h of in vitro culture for oocytes vitrified at the MII stage. The multistep equilibration method allowed better survival rates after thawing for CE oocytes when vitrified at GV stage (61.3% vs 37.1%) and for CC oocytes when vitrified at MII stage (83.3% vs 61.4%)
A 3-steps equilibration method was compared to a single-step one in vitrification of cumulus-expanded (CE) and cumulus-compacted (CC) horse oocytes, both in GV and MII stage, obtained from ovaries of slaughtered mares. After thawing oocyte viability was assessed by nuclear maturation rate after in vitro culture for oocytes vitrified at the GV stage and by the presence of an intact zona pellucida and membrane after 2 h of in vitro culture for oocytes vitrified at the MII stage. The multistep equilibration method allowed better survival rates after thawing for CE oocytes when vitrified at GV stage (61.3% vs 37.1%) and for CC oocytes when vitrified at MII stage (83.3% vs 61.4%).
VITRIFICATION OF GV AND IVM HORSE OOCYTES WITH TWO DIFFERENT EQUILIBRATION METHODS / Carboni, S; Rosati, Irma; Maclellan, Lj; Ariu, F; Bogliolo, Luisa; Zedda, Maria Teresa; Pau, Salvatore; Carnevale, Em; Ledda, Sergio. - (2012), pp. 12-13. (Intervento presentato al convegno Proceedings of 10th Congress of Italian Society of Animal Reproduction 1st Balkan Conference on Biology and Reproduction in Farm Animals and in Aquaculture tenutosi a Tirana nel 23-25 May, 2012) [10.4488/2012.SIRA.A.005].
VITRIFICATION OF GV AND IVM HORSE OOCYTES WITH TWO DIFFERENT EQUILIBRATION METHODS
ROSATI, Irma;BOGLIOLO, Luisa;ZEDDA, Maria Teresa;PAU, Salvatore;LEDDA, Sergio
2012-01-01
Abstract
A 3-steps equilibration method was compared to a single-step one in vitrification of cumulus-expanded (CE) and cumulus-compacted (CC) horse oocytes, both in GV and MII stage, obtained from ovaries of slaughtered mares. After thawing oocyte viability was assessed by nuclear maturation rate after in vitro culture for oocytes vitrified at the GV stage and by the presence of an intact zona pellucida and membrane after 2 h of in vitro culture for oocytes vitrified at the MII stage. The multistep equilibration method allowed better survival rates after thawing for CE oocytes when vitrified at GV stage (61.3% vs 37.1%) and for CC oocytes when vitrified at MII stage (83.3% vs 61.4%)I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
