In vitro matured horse oocytes were cryopreserved by vitrification using the Minima! Essential Volume Technique (MEMT) using as device the Cryotops. MII oocytes derived from expanded ( +) and compacted cumuls cells (-) were divided for the vitrification procedures as follow: A) Oocytes were exposed for 3' in TCM 199 + 20% FSC with 7.5% EG + 7.5% DMSO; 20-30 " in TCM 199 + 20% FSC + 16.5% EG + 16.5% DMSO + 0.5M saccarosio(V1); loaded with 2ul ofV1 on the cryotop and pluged directly in the liquid nitrogen; B) oocytes were incubated in TCM 199 + 20% FCS + 10% EG + 10% DMSO per 30"; 20" in TCM 199 + 20% FCS + 20% EG + 20% DMSO + 0.25 M Sucrose , loaded in the cryotop(2Jll) and pluged in the liquid nitrogen. Thawing was performed washing the oocytes in TCM 199 + 20% FCS with decreasing sucrose concentrations (1.25M, 0.62 M, 0.31 M). Surviving rate of the oocyte was no t statistically different in expanded and compacted oocytes and in A and B systems ranging between 40.9% and 57.1 %. After ICSI we observed lower fertilization rate in vitrified oocytes compared to the contro! group

VITRIFICAZIONE di oociti MII di cavallo mediante tecnica Minimal Essential Volume(cryotop) / Bogliolo, Luisa; Ariu, F; Zedda, Maria Teresa; Pau, Salvatore; Ledda, S.. - (2004), pp. 87-89. ((Intervento presentato al convegno Atti II Congresso Nazionale Società Italiana Riproduzione Animale tenutosi a torino nel 9-10 giugno 2004.

VITRIFICAZIONE di oociti MII di cavallo mediante tecnica Minimal Essential Volume(cryotop)

BOGLIOLO, Luisa;ZEDDA, Maria Teresa;PAU, Salvatore;LEDDA S.
2004

Abstract

In vitro matured horse oocytes were cryopreserved by vitrification using the Minima! Essential Volume Technique (MEMT) using as device the Cryotops. MII oocytes derived from expanded ( +) and compacted cumuls cells (-) were divided for the vitrification procedures as follow: A) Oocytes were exposed for 3' in TCM 199 + 20% FSC with 7.5% EG + 7.5% DMSO; 20-30 " in TCM 199 + 20% FSC + 16.5% EG + 16.5% DMSO + 0.5M saccarosio(V1); loaded with 2ul ofV1 on the cryotop and pluged directly in the liquid nitrogen; B) oocytes were incubated in TCM 199 + 20% FCS + 10% EG + 10% DMSO per 30"; 20" in TCM 199 + 20% FCS + 20% EG + 20% DMSO + 0.25 M Sucrose , loaded in the cryotop(2Jll) and pluged in the liquid nitrogen. Thawing was performed washing the oocytes in TCM 199 + 20% FCS with decreasing sucrose concentrations (1.25M, 0.62 M, 0.31 M). Surviving rate of the oocyte was no t statistically different in expanded and compacted oocytes and in A and B systems ranging between 40.9% and 57.1 %. After ICSI we observed lower fertilization rate in vitrified oocytes compared to the contro! group
VITRIFICAZIONE di oociti MII di cavallo mediante tecnica Minimal Essential Volume(cryotop) / Bogliolo, Luisa; Ariu, F; Zedda, Maria Teresa; Pau, Salvatore; Ledda, S.. - (2004), pp. 87-89. ((Intervento presentato al convegno Atti II Congresso Nazionale Società Italiana Riproduzione Animale tenutosi a torino nel 9-10 giugno 2004.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/63650
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