Cold shock can injure spermatozoa in different levels and can alter spermatozoa functional integrity hence reducing their fertilizing ability. A significant reduction in the level of spermatozoa antioxidants has been reported as one of the causes of the enhanced susceptibility of these cells to peroxidative injuries after cryopreservation. The aim of this work was to investigate whether there are specie‐specific differences in the total antioxidant capacity and total thiols and GSH content in seminal plasma and sperm cell extract among boar, goat and ram.   Samples were processed rapidly and kept frozen at − 80 °C un�l assayed. TAC of seminal plasma and sperm cell extract was determined using the TEAC assay as described by Re et al.  (1999). Briefly, the TEAC assay measures the relative ability of circulating antioxidants to scavenge 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐ sulfonic acid) (ABTS) radical cations (ABTS+) in comparison with the antioxidant capacity of Trolox standards. The absorbance was measured at 734 nm and the antioxidant activity was expressed as Trolox equivalent. GSH and total thiols content were determined in sperm cell extract by spectrophotometric method using dithionitrobenzoic acid (DTNB) by Ellman's assay as described by Patsoukis and Georgiou (2004).   Obtained results showed that GSH and total thiols content were significantly lower in swine compared to small ruminants (p<0.01). On the other hand, both seminal plasma and sperm cell extract showed a significantly higher TAC in swine compared to small ruminants. Within the same species, seminal plasma proved to have a higher TAC compared to sperm cell extract (p<0.05). This results suggest that boar spermatozoa and seminal plasma may be endorsed with higher enzymatic antioxidant defense or with non‐enzymatic compounds other than thiols, such as alpha‐tocopherol, beta‐ carotene,    ascorbate, or urate. Further studies are needed to characterize the concentration of the full spectrum of possible anti‐oxidants in these three species. This finding can be applied to develop protocol to optimize semen cryopreservation protocols, but may also have an application in developing therapeutic protocols for male infertility related to a decrease in sperm antioxidant defense system.  

Evaluation of antioxidant capacity in seminal plasma and sperm cell extract of boar, ram and goat / Addis D; Pasciu V; Succu S; Torres-Rovira L; Manca ME; Chelucci S; Leoni GG; Berlinguer F; Naitana S. - LXVII:(2013), pp. 136-136. ((Intervento presentato al convegno Convegno Nazionale SISVet 2013 tenutosi a Brescia nel 17-19 settembre.

Evaluation of antioxidant capacity in seminal plasma and sperm cell extract of boar, ram and goat.

SUCCU, Sara;LEONI, Giovanni Giuseppe;Berlinguer F;NAITANA, Salvatore
2013

Abstract

Cold shock can injure spermatozoa in different levels and can alter spermatozoa functional integrity hence reducing their fertilizing ability. A significant reduction in the level of spermatozoa antioxidants has been reported as one of the causes of the enhanced susceptibility of these cells to peroxidative injuries after cryopreservation. The aim of this work was to investigate whether there are specie‐specific differences in the total antioxidant capacity and total thiols and GSH content in seminal plasma and sperm cell extract among boar, goat and ram.   Samples were processed rapidly and kept frozen at − 80 °C un�l assayed. TAC of seminal plasma and sperm cell extract was determined using the TEAC assay as described by Re et al.  (1999). Briefly, the TEAC assay measures the relative ability of circulating antioxidants to scavenge 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐ sulfonic acid) (ABTS) radical cations (ABTS+) in comparison with the antioxidant capacity of Trolox standards. The absorbance was measured at 734 nm and the antioxidant activity was expressed as Trolox equivalent. GSH and total thiols content were determined in sperm cell extract by spectrophotometric method using dithionitrobenzoic acid (DTNB) by Ellman's assay as described by Patsoukis and Georgiou (2004).   Obtained results showed that GSH and total thiols content were significantly lower in swine compared to small ruminants (p<0.01). On the other hand, both seminal plasma and sperm cell extract showed a significantly higher TAC in swine compared to small ruminants. Within the same species, seminal plasma proved to have a higher TAC compared to sperm cell extract (p<0.05). This results suggest that boar spermatozoa and seminal plasma may be endorsed with higher enzymatic antioxidant defense or with non‐enzymatic compounds other than thiols, such as alpha‐tocopherol, beta‐ carotene,    ascorbate, or urate. Further studies are needed to characterize the concentration of the full spectrum of possible anti‐oxidants in these three species. This finding can be applied to develop protocol to optimize semen cryopreservation protocols, but may also have an application in developing therapeutic protocols for male infertility related to a decrease in sperm antioxidant defense system.  
Evaluation of antioxidant capacity in seminal plasma and sperm cell extract of boar, ram and goat / Addis D; Pasciu V; Succu S; Torres-Rovira L; Manca ME; Chelucci S; Leoni GG; Berlinguer F; Naitana S. - LXVII:(2013), pp. 136-136. ((Intervento presentato al convegno Convegno Nazionale SISVet 2013 tenutosi a Brescia nel 17-19 settembre.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/55600
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