New findings on phytoplasmas-affected Auchenorrhyncha populations in Sardinian vineyards

Epidemiological research was carried out in two vineyards affected by "Bois noir" (BN). Auchenorrhyncha potential vectors of BN, were monitored periodically between May to November 2004 in a vineyard and between May to June 2005 in the other. Auchenorrhyncha samples were tested to assess phytoplasmas presence using PCR and RFLP.Euscelis ttneolatuswas positive to 16SrI-C ("Clover phyllody" reference strain) in 2004 while, in 2005, at the preimaginal age, at the 16SrXII -A ("Stolbur" reference strain) phytoplasmas.Exitianus taeniaticepsacquired 16SrI-B ("Maryland aster yellow" reference strain), 16SrV-A ("Elm yellow" reference strain) and 16SrX-C ("Pear decline" reference strain) phytoplasmas in 2004. Resulted news host of phytoplasmas:Psammotettix alienuswas positive to 16SrI-B, 16SrV-A, 16SrX- A phytoplasmas ("Apple proliferation" reference strain) in 2004 and to 16SrXII -A in 2005.K. lineolatusandP. alienusfor 16SrXII -AE. taeniaticepsfor 16SrV-A 16SrX-C andP. alienusfor 16SrV-A and 16SrX-A Researches on the effective epidemiological role ofK. lineolatusandP. alienusin BN are in progress.