Development of a real-time PCR for detection of Mycoplasma agalactiae in bulk tank milk samples and epidemiology of infection in Sardinia

DEVELOPMENT OF A REAL-TIME PCR FOR DETECTION OF MYCOPLASMA AGALACTIAE IN BULK TANK MILK SAMPLES AND EPIDEMIOLOGY OF INFECTION IN SARDINIA Carta T. [1], Mannu F.[2], Fadda M.[1], Ibba I.[3], Muggianu D.[3], Turrini F.[2], Pittau M.[1], Chessa B.[1] [1]Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari ~ Sassari ~ Italy, [2]Nurex s.r.l. ~ Sassari ~ Italy, [3]Associazione Regionale Allevatori Sardegna (ARAS) ~ Oristano ~ Italy In this work the Mycoplasma agalactiae p48 gene was used as a diagnostic marker for contagious agalactia (CA) of sheep and goats by Real-Time PCR. The p48 gene encodes an invariable, constantly expressed, immunodominant surface lipoprotein belongs to the basic membrane protein family. The Real-Time PCR test based on p48 resulted specific and sensible. The test performance were evaluated on bulk tank milk samples collected from 1064 ovine and 66 goat farms in sardinian region. 4.8% of sheep farms and 4.5 % of goat farms tested positive. Our results showed that the test based on the p48 gene can be used on bulk tank milk for detection and epidemiological surveillance of Mycoplasma agalactiae infections.