Objectives: To study the interfacial characteristics of an experimental antibacterial adhesive system, ABF (Kuraray, Osaka, Japan) applied to sclerotic dentine and its effect on residual bacterial colonies, using TEM. Methods: Carious molars (no = 8) scheduled for extraction for orthodontic purposes (age range 16 to 30 years), were selected for this study. Cavity preparation removed all the superficial (soft/yellowish) caries, producing a cavity floor of hard and stained dentine. The experimental primer, containing MDPB synthesized from a quaternary ammonium compound, was applied to the cavity floor for 20 sec and light cured for 20 sec. A microfilled resin-based composite restoration was then placed and cured. The teeth were extracted and processed for TEM. Results: the interdiffusion in dentine showed a very thin hybrid layer. Tubules were blocked by sclerotic casts, short resin tags containing a core of crystallites, and bacterial colonies were immobilized in the resin network. At high magnification, bacterial cells membranes appeared erupted and microfillers particles were seen in their cytoplasm. Conclusions: Although a classic hybrid layer was absent within the sclerotic cavity, ABF was incorporated into the inter diffusion. The capacity to surround and immobilize the contained bacterial colonies has relevant potential for prevention of secondary caries.

Resin adhesion to sclerotic dentin: An in vivo TEM study of a new antibacterial system / Milia, Egle Patrizia; Campus, Guglielmo Giuseppe; Santini, A.. - In: JOURNAL OF DENTAL RESEARCH. - ISSN 0022-0345. - B:(2003), p. B255. ((Intervento presentato al convegno 81st General Session of the IADR tenutosi a Goteborg, Sweden nel June 25-28, 2003.

Resin adhesion to sclerotic dentin: An in vivo TEM study of a new antibacterial system

MILIA, Egle Patrizia;CAMPUS, Guglielmo Giuseppe;
2003

Abstract

Objectives: To study the interfacial characteristics of an experimental antibacterial adhesive system, ABF (Kuraray, Osaka, Japan) applied to sclerotic dentine and its effect on residual bacterial colonies, using TEM. Methods: Carious molars (no = 8) scheduled for extraction for orthodontic purposes (age range 16 to 30 years), were selected for this study. Cavity preparation removed all the superficial (soft/yellowish) caries, producing a cavity floor of hard and stained dentine. The experimental primer, containing MDPB synthesized from a quaternary ammonium compound, was applied to the cavity floor for 20 sec and light cured for 20 sec. A microfilled resin-based composite restoration was then placed and cured. The teeth were extracted and processed for TEM. Results: the interdiffusion in dentine showed a very thin hybrid layer. Tubules were blocked by sclerotic casts, short resin tags containing a core of crystallites, and bacterial colonies were immobilized in the resin network. At high magnification, bacterial cells membranes appeared erupted and microfillers particles were seen in their cytoplasm. Conclusions: Although a classic hybrid layer was absent within the sclerotic cavity, ABF was incorporated into the inter diffusion. The capacity to surround and immobilize the contained bacterial colonies has relevant potential for prevention of secondary caries.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/167328
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