NUCLEAR CONFIGURATION AND LIPIDS DISTRIBUTION OF IMMATURE CANINE OOCYTES FROM DIFFERENT OESTROUS STAGE.

"In the present study we observed chromatin configuration and lipid. distribution in immature canine oocytes (GV) of different diameters(110-120, >120μm). collected from ovaries of bitches at different stages of the oestrous cycle(anoestrus,. follicular phase and luteal phase). The immature oocytes were stained with 1μg\/ml Nile. Red and 0.1 mg\/mL bis-benzimide to analyze lipids and GV chromatin configuration,. respectively. We classified the GV stages (four types: GV-I to GV-IV) as previously. described analyzing the different pattern of chromatin configuration and lipid. distribution in the different reproductive stages:-Anoestrus phase, GV-I and GV-II. oocytes of 110-120μm showed small lipid droplets in the cortical area and larger lipid. droplets around the nucleus while GV-I and GV-II >120μm had lipid droplets. uniformly distributed in the cytoplasm. -Follicular phase, GV-II e GV-IV present lipid. droplets uniformly distributed in the cytoplasm irrespective the oocyte diameter(110-. 120μm and >120μm). -Luteal phase, GV-II and GV-III(110-12μm) showed lipid. droplets with a higher concentration in the cortical area while for those >120μm(GV-II. and GV- III) the lipid droplets were a highly concentrated around the nuclear membrane. and in the cortical area. The different chromatin configuration and lipid distribution. could be related to the different developmental competence and represent a parameter to. assess the oocyte meiotic competence.. "