"We report on the development of 10 microsatellites isolated from Opuntia ficus-indica. To evaluate the degree of polymorphism of these markers, a range of cultivars from the germplasm collection established at the Experimental Station of the University of Sassari has been tested. DNA from 12 Opuntia species was also used to test transferability of the developed primers. The level of polymorphism and the relatively high number of alleles detected ranging from 6 to 22 suggest that these markers can be used for both inter- and intra-specific studies, as well as to analyze the polyploidy occurrence in Opuntia."
Identification of Microsatellite Loci in Opuntia spp. and Their Characterization in Cultivars and Species / Erre, P; Nieddu, Giovanni; Chessa, Innocenza. - In: ACTA HORTICULTURAE. - ISSN 0567-7572. - 918:(2011), pp. 327-332. (Intervento presentato al convegno 28th International Horticultural Congress on Science and Horticulture for People (IHC) / International Symposium on Plant Protection / 3rd International Symposium on Plant Genetic Resources).
Identification of Microsatellite Loci in Opuntia spp. and Their Characterization in Cultivars and Species
NIEDDU, Giovanni;CHESSA, Innocenza
2011-01-01
Abstract
"We report on the development of 10 microsatellites isolated from Opuntia ficus-indica. To evaluate the degree of polymorphism of these markers, a range of cultivars from the germplasm collection established at the Experimental Station of the University of Sassari has been tested. DNA from 12 Opuntia species was also used to test transferability of the developed primers. The level of polymorphism and the relatively high number of alleles detected ranging from 6 to 22 suggest that these markers can be used for both inter- and intra-specific studies, as well as to analyze the polyploidy occurrence in Opuntia."I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
