Background: Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC. Materials & methods: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 μm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 μm) for CE analysis, monitoring the fluorescence of derivatives. Results: Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ. Conclusion: This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.
Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC / Sotgia, Salvatore; Arru, D; Sotgiu, E; Mangoni, Arduino Aleksander; Forteschi, Mauro; Pintus, Gianfranco; Carru, Ciriaco; Zinellu, Angelo. - In: BIOANALYSIS. - ISSN 1757-6180. - 8:9(2016), pp. 945-951. [10.4155/bio-2015-0002]
Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC
SOTGIA, Salvatore
;PINTUS, Gianfranco;CARRU, Ciriaco;ZINELLU, Angelo
2016-01-01
Abstract
Background: Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC. Materials & methods: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 μm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 μm) for CE analysis, monitoring the fluorescence of derivatives. Results: Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ. Conclusion: This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
