The aim of our work was to assess the effect of cryoprotectants and vitrification procedures on prepubertal ovine oocytes. Prepubertal and adult COCs were matured in vitro and exposed to cryoprotectants (EXP) or vitrified/warmed (VIT) using the cryoloop as cryodevice. After treatment, we evaluated in vitro the viability and development and chromatin alterations. Prepubertal and adult oocytes did not differ (p > 0.05) in degeneration and cleavage rates both in EXP and VIT groups. In the EXP group, prepubertal blastocyst output was lower (p < 0.05) than in the adults, while it did not differ in VIT group. Chromatin alterations were more evident in prepubertal than in adult oocytes both in EXP and VIT groups (p < 0.01). In conclusion, prepubertal ovine oocytes show a high sensitivity to vitrification procedures and their use for cryobanking require more studies.
The aim of our work was to assess the effect of cryoprotectants and vitrification procedures on prepubertal ovine oocytes. Prepubertal and adult COCs were matured in vitro and exposed to cryoprotectants (EXP) or vitrified/warmed (VIT) using the cryoloop as cryodevice. After treatment, we evaluated in vitro the viability and development and chromatin alterations. Prepubertal and adult oocytes did not differ (p > 0.05) in degeneration and cleavage rates both in EXP and VIT groups. In the EXP group, prepubertal blastocyst output was lower (p < 0.05) than in the adults, while it did not differ in VIT group. Chromatin alterations were more evident in prepubertal than in adult oocytes both in EXP and VIT groups (p < 0.01). In conclusion, prepubertal ovine oocytes show a high sensitivity to vitrification procedures and their use for cryobanking require more studies.
Prepubertal and adult ovine oocytes show different sensitivity to vitrification procedures / Succu, Sara; Leoni, Giovanni Giuseppe; Madeddu, M; Satta, Valentina; Bogliolo, Luisa; Bebbere, Daniela; Spezzigu, Antonio; Tedde, A; Berlinguer, Fiammetta; Ledda, Sergio; Naitana, Salvatore. - In: COMPTES RENDUS DE L'ACADÉMIE BULGARE DES SCIENCES. - ISSN 1310-1331. - 62(9):9(2009), pp. 1119-1124.
Prepubertal and adult ovine oocytes show different sensitivity to vitrification procedures
SUCCU, Sara;LEONI, Giovanni Giuseppe;SATTA, Valentina;BOGLIOLO, Luisa;BEBBERE, Daniela;SPEZZIGU, ANTONIO;BERLINGUER, Fiammetta;LEDDA, Sergio;NAITANA, Salvatore
2009-01-01
Abstract
The aim of our work was to assess the effect of cryoprotectants and vitrification procedures on prepubertal ovine oocytes. Prepubertal and adult COCs were matured in vitro and exposed to cryoprotectants (EXP) or vitrified/warmed (VIT) using the cryoloop as cryodevice. After treatment, we evaluated in vitro the viability and development and chromatin alterations. Prepubertal and adult oocytes did not differ (p > 0.05) in degeneration and cleavage rates both in EXP and VIT groups. In the EXP group, prepubertal blastocyst output was lower (p < 0.05) than in the adults, while it did not differ in VIT group. Chromatin alterations were more evident in prepubertal than in adult oocytes both in EXP and VIT groups (p < 0.01). In conclusion, prepubertal ovine oocytes show a high sensitivity to vitrification procedures and their use for cryobanking require more studies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
