In order to investigate if the melatonin receptor 1A (MTNR1A) and kisspeptin (KiSS-1) genes influence the reproductive response to melatonin treatment, 510 Sarda ewe lambs were divided into groups C (control) and M; GroupM received one melatonin implant (18 mg). After 35 days rams were introduced for 40 days and subsequent lambing dates and number of newborns were recorded. The MTNR1A gene Exon II and KiSS-1 gene Exon I were amplified and genotyped by restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism analysis. Two single nucleotide polymorphisms (SNPs; C606T and G612A) in MTNR1A and one (G1035A) in KiSS-1 were found. The most frequent genotypes were G/G (63%) and C/C (53%) for MTNR1A and G/G (92%) for KiSS-1. Treated animals showed a higher lambing rate (P,0.05) and an advanced lambing date (P,0.05) compared with controls. The three SNPs did not influence the onset of reproductive activity. The majority of the G/G animals of Group M lambed before 190 days after ram introduction (P,0.05), while in Group C a higher number of G/G animals lambed after this date. Data revealed the positive effect of melatonin treatment on the time of first conception in ewe lambs and highlighted that the G/G genotype of the MTNR1A gene is able to influence the reproductive response to melatonin treatment.

Influences of melatonin treatment, MTNR1A and KiSS-1 genes polymorphisms on first conception in Sarda ewe lambs / Luridiana, Sebastiano; Mura, Maria Consuelo; Daga, Cinzia; Cosso, G.; Bodano, S.; Farci, Federico; Zidda, F.; Carcangiu, Vincenzo. - In: REPRODUCTION FERTILITY AND DEVELOPMENT. - ISSN 1031-3613. - 28:(2016), pp. 750-756. [10.1071/RD14120]

Influences of melatonin treatment, MTNR1A and KiSS-1 genes polymorphisms on first conception in Sarda ewe lambs.

LURIDIANA, Sebastiano;MURA, Maria Consuelo;DAGA, Cinzia;FARCI, Federico;CARCANGIU, Vincenzo
2016-01-01

Abstract

In order to investigate if the melatonin receptor 1A (MTNR1A) and kisspeptin (KiSS-1) genes influence the reproductive response to melatonin treatment, 510 Sarda ewe lambs were divided into groups C (control) and M; GroupM received one melatonin implant (18 mg). After 35 days rams were introduced for 40 days and subsequent lambing dates and number of newborns were recorded. The MTNR1A gene Exon II and KiSS-1 gene Exon I were amplified and genotyped by restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism analysis. Two single nucleotide polymorphisms (SNPs; C606T and G612A) in MTNR1A and one (G1035A) in KiSS-1 were found. The most frequent genotypes were G/G (63%) and C/C (53%) for MTNR1A and G/G (92%) for KiSS-1. Treated animals showed a higher lambing rate (P,0.05) and an advanced lambing date (P,0.05) compared with controls. The three SNPs did not influence the onset of reproductive activity. The majority of the G/G animals of Group M lambed before 190 days after ram introduction (P,0.05), while in Group C a higher number of G/G animals lambed after this date. Data revealed the positive effect of melatonin treatment on the time of first conception in ewe lambs and highlighted that the G/G genotype of the MTNR1A gene is able to influence the reproductive response to melatonin treatment.
2016
Influences of melatonin treatment, MTNR1A and KiSS-1 genes polymorphisms on first conception in Sarda ewe lambs / Luridiana, Sebastiano; Mura, Maria Consuelo; Daga, Cinzia; Cosso, G.; Bodano, S.; Farci, Federico; Zidda, F.; Carcangiu, Vincenzo. - In: REPRODUCTION FERTILITY AND DEVELOPMENT. - ISSN 1031-3613. - 28:(2016), pp. 750-756. [10.1071/RD14120]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/60397
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