Background: Chromosome 10q25-q26 has been strongly correlated to endometrial tumorigenesis. A novel human gene, CASC2, has previously been identified at chromosome 10q26. One out of the three alternative transcripted forms, CASC2a, has been demonstrated to be mutated at a low frequency in endometrial cancer (EC). In this study, the role of the CASC2a gene in cancer has been further defined. Materials and Methods: Tumour and corresponding normal tissues were analysed for CASC2a mRNA expression by real-time RT-PCR and mutation status by PCR-based approaches. Results: A significantly decreased level of CASC2a transcripts was observed in 13/17 (76%) EC tissues, as well as in 6/9 (67%) colorectal cancers. Exogenous expression of CASC2a in undifferentiated AN3CA endometrial cancer cells inhibited cellular growth in anchorage-independent growth assays. Finally, infrequent CASC2a mutations were able to impair the gene function. Conclusion: Altogether, our findings strongly suggest that CA5C2a may act as a tumour suppressor gene, with both epigenetic and genetic alterations concurring to gene inactivation. Down-regulation of CASC2a may provide a growth advantage in EC cells.

CASC2a gene is down-regulated in endometrial cancer / Baldinu, Paola; Cossu, A; Manca, A; Satta, Mp; Sini, Mc; Palomba, G; Dessole, S; Cherchi, P; Mara, L; Tanda, F; Palmieri, G. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - 27:1A(2007), pp. 235-243.

CASC2a gene is down-regulated in endometrial cancer

BALDINU, Paola;COSSU A;DESSOLE S;CHERCHI P;TANDA F;PALMIERI G
2007-01-01

Abstract

Background: Chromosome 10q25-q26 has been strongly correlated to endometrial tumorigenesis. A novel human gene, CASC2, has previously been identified at chromosome 10q26. One out of the three alternative transcripted forms, CASC2a, has been demonstrated to be mutated at a low frequency in endometrial cancer (EC). In this study, the role of the CASC2a gene in cancer has been further defined. Materials and Methods: Tumour and corresponding normal tissues were analysed for CASC2a mRNA expression by real-time RT-PCR and mutation status by PCR-based approaches. Results: A significantly decreased level of CASC2a transcripts was observed in 13/17 (76%) EC tissues, as well as in 6/9 (67%) colorectal cancers. Exogenous expression of CASC2a in undifferentiated AN3CA endometrial cancer cells inhibited cellular growth in anchorage-independent growth assays. Finally, infrequent CASC2a mutations were able to impair the gene function. Conclusion: Altogether, our findings strongly suggest that CA5C2a may act as a tumour suppressor gene, with both epigenetic and genetic alterations concurring to gene inactivation. Down-regulation of CASC2a may provide a growth advantage in EC cells.
2007
CASC2a gene is down-regulated in endometrial cancer / Baldinu, Paola; Cossu, A; Manca, A; Satta, Mp; Sini, Mc; Palomba, G; Dessole, S; Cherchi, P; Mara, L; Tanda, F; Palmieri, G. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - 27:1A(2007), pp. 235-243.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/57249
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