Inpiego di differenti concentrazioni di CPA e tempi di esposizione per la vitrificazione di oociti IVM di gatto.

Experiments were carried out to test viability and meiotic competence of immature and mature cat oocytes vitrified by the MEMT with cryotops using two different methods: A) incubation with 10% EG+10% DMSO (30sec.); 20% EG+ 20%DMSO+ 0.25M sucrose (20 sec.) B) incubation with 7.5 % EG +7.5 % DMSO (3 min.); 15 % EG+ 15 % DMSO+ 0.5M sucrose (1 min.). In Exp.1 immature cat oocyte were vitrified immediately after collection without cumulus cells. After thawing oocytes were matured in vitro for 24 h with additional fresh cumulus cells. The results indicate that system B induced a significantly higher viability and developmental competence to MII compared with system A. In Exp.2, in vitro mature cat oocytes were vitrified with system A and B. Results showed that survival rate of the oocyte was statistically different after vitrification in A (8.7% ) and B (80.5%) systems.