The antimicrobial activity of tetracyc line hydrochloride (OTC) and cinnamon ( Cinnamomum zeylanicum Nees) essential oil (CEO) was evaluated against six different isolates of Paenibacillus larvae (White), the causal agent of Am erican Foulbrood (AFB) disease in honey bee colonies. The bacteria isolates were collected from different localit ies of Argentine. Minimal inhibitory concentration (MIC) in MYT broth by the tube dilution method was evaluated fo r each substance and for the combinations of both antimicrobials using Krogstad and Moellering technique in order to establish the possible synergistic effects between these substances. OTC me an MIC values were of 3.67 ± 1.80 µg/ml, while the mean MIC values obtained for CEO were of 41.67±19.17 µg/ml. An inhibitory synergetic effect between these substances was observed with FIC index < 1 on 50% of the on P. larvae isolates.
Susceptibility of Paenibacillus larvae isolates to a tetracycline hydrochloride and Cinnamon (Cinnamomum zeylanicum) essential oil mixture / Gende, Lb; Fernandez, N; Buffa, F.; Ruiu, Luca; Satta, Alberto; Fritz, R; Eguaras, Mj; Floris, Ignazio. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - 63:2(2010), pp. 247-250.
Susceptibility of Paenibacillus larvae isolates to a tetracycline hydrochloride and Cinnamon (Cinnamomum zeylanicum) essential oil mixture
RUIU, Luca;SATTA, Alberto;FLORIS, Ignazio
2010-01-01
Abstract
The antimicrobial activity of tetracyc line hydrochloride (OTC) and cinnamon ( Cinnamomum zeylanicum Nees) essential oil (CEO) was evaluated against six different isolates of Paenibacillus larvae (White), the causal agent of Am erican Foulbrood (AFB) disease in honey bee colonies. The bacteria isolates were collected from different localit ies of Argentine. Minimal inhibitory concentration (MIC) in MYT broth by the tube dilution method was evaluated fo r each substance and for the combinations of both antimicrobials using Krogstad and Moellering technique in order to establish the possible synergistic effects between these substances. OTC me an MIC values were of 3.67 ± 1.80 µg/ml, while the mean MIC values obtained for CEO were of 41.67±19.17 µg/ml. An inhibitory synergetic effect between these substances was observed with FIC index < 1 on 50% of the on P. larvae isolates.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
