This work was undertaken to determine whether Acanthamoeba could play a role in the survival and transmission of coxsackieviruses and focused on in vitro interactions between Acanthamoeba castellanii and coxsackie B3 viruses (CVB-3). Residual virus titer evaluations and immunofluorescence experiments revealed a remarkable CVB-3 adsorption on amoeba surfaces and accumulation inside cells. The survival of viruses was independent of the dynamics of amoeba replication and encystment. In addition, our results indicated that virus-infected amoebas can release infectious viruses during interaction with human macrophages. On the basis of these data, Acanthamoeba appears to be a potential promoter of the survival of coxsackieviruses and their transmission to human hosts.

Acanthamoeba castellanii promotion of the in vitro survival and transmission of Coxsackie-B-3 viruses / Mattana, Antonella; Serra, Caterina; E., Mariotti; G., Delogu; Fiori, Pier Luigi; P., Cappuccinelli. - In: EUKARYOTIC CELL. - ISSN 1535-9778. - 5:(2006), pp. 665-671.

Acanthamoeba castellanii promotion of the in vitro survival and transmission of Coxsackie-B-3 viruses

MATTANA, Antonella;SERRA, Caterina;FIORI, Pier Luigi;
2006-01-01

Abstract

This work was undertaken to determine whether Acanthamoeba could play a role in the survival and transmission of coxsackieviruses and focused on in vitro interactions between Acanthamoeba castellanii and coxsackie B3 viruses (CVB-3). Residual virus titer evaluations and immunofluorescence experiments revealed a remarkable CVB-3 adsorption on amoeba surfaces and accumulation inside cells. The survival of viruses was independent of the dynamics of amoeba replication and encystment. In addition, our results indicated that virus-infected amoebas can release infectious viruses during interaction with human macrophages. On the basis of these data, Acanthamoeba appears to be a potential promoter of the survival of coxsackieviruses and their transmission to human hosts.
2006
Acanthamoeba castellanii promotion of the in vitro survival and transmission of Coxsackie-B-3 viruses / Mattana, Antonella; Serra, Caterina; E., Mariotti; G., Delogu; Fiori, Pier Luigi; P., Cappuccinelli. - In: EUKARYOTIC CELL. - ISSN 1535-9778. - 5:(2006), pp. 665-671.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/46960
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