Human dental pulp stem cells (hDPSCs) have shown relevant potential for cell therapy in the orthopedic and odontoiatric fields. The optimization of their osteogenic potential is currently a major challenge. Vascular endothelial growth factor A (VEGF A) has been recently reported to act as a major conductor of osteogenesis in vitro and in vivo. Here, we attempted to prime endogenous VEGF A expression without the need for viral vector mediated gene transfer technologies. We show that hDPSCs exposure to a mixture of hyaluronic, butyric, and retinoic acids (HA + BU + RA) induced the transcription of a gene program of osteogenesis and the acquirement of an osteogenic lineage. Such response was also elicited by cell exposure to melatonin, a pleiotropic agent that recently emerged as a remarkable osteogenic inducer. Interestingly, the commitment to the osteogenic fate was synergistically enhanced by the combinatorial exposure to a conditioned medium containing both melatonin and HA + BU + RA. These in vitro results suggest that in vivo osteogenesis might be improved and further studies are needed.

Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids / Maioli, Margherita; Basoli, V; Santaniello, S; Cruciani, S; Delitala, Ap; Pinna, R; Milia, Egle Patrizia; Grillari Voglauer, R; Fontani, V; Rinaldi, S; Muggironi, R; Pigliaru, G; Ventura, C.. - In: STEM CELLS INTERNATIONAL. - ISSN 1687-9678. - 2016:(2016), pp. ID 2056416,.1-ID 2056416,.8. [10.1155/2016/2056416]

Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids

MAIOLI, Margherita;Cruciani S;Delitala AP;MILIA, Egle Patrizia;
2016-01-01

Abstract

Human dental pulp stem cells (hDPSCs) have shown relevant potential for cell therapy in the orthopedic and odontoiatric fields. The optimization of their osteogenic potential is currently a major challenge. Vascular endothelial growth factor A (VEGF A) has been recently reported to act as a major conductor of osteogenesis in vitro and in vivo. Here, we attempted to prime endogenous VEGF A expression without the need for viral vector mediated gene transfer technologies. We show that hDPSCs exposure to a mixture of hyaluronic, butyric, and retinoic acids (HA + BU + RA) induced the transcription of a gene program of osteogenesis and the acquirement of an osteogenic lineage. Such response was also elicited by cell exposure to melatonin, a pleiotropic agent that recently emerged as a remarkable osteogenic inducer. Interestingly, the commitment to the osteogenic fate was synergistically enhanced by the combinatorial exposure to a conditioned medium containing both melatonin and HA + BU + RA. These in vitro results suggest that in vivo osteogenesis might be improved and further studies are needed.
2016
Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids / Maioli, Margherita; Basoli, V; Santaniello, S; Cruciani, S; Delitala, Ap; Pinna, R; Milia, Egle Patrizia; Grillari Voglauer, R; Fontani, V; Rinaldi, S; Muggironi, R; Pigliaru, G; Ventura, C.. - In: STEM CELLS INTERNATIONAL. - ISSN 1687-9678. - 2016:(2016), pp. ID 2056416,.1-ID 2056416,.8. [10.1155/2016/2056416]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/45905
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