Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in ruminants, has been linked as a possible risk factor for human multiple sclerosis. In the current study we investigated the adjuvant effect of MAP on experimental autoimmune encephalomyelitis (EAE). Groups of C57BL/6 mice were actively immunized with myelin oligodendrocyte glycoprotein (MOG) 35–55 peptide emulsified in incomplete Freund's adjuvant modified containing heat-killed MAP (MIFA). MOG-MIFA immunized mice showed an early disease onset and more severe clinical scores in comparison with MOG-CFA immunized mice, demonstrating for the first time the adjuvant effect of MAP on EAE development.
Adjuvant and antigenic properties of Mycobacterium avium subsp. paratuberculosis on experimental autoimmune encephalomyelitis / Cossu, D.; Yokoyama, K.; Sakanishi, T.; Momotani, E.; Hattori, N.. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 330:(2019), pp. 174-177. [10.1016/j.jneuroim.2019.01.013]
Adjuvant and antigenic properties of Mycobacterium avium subsp. paratuberculosis on experimental autoimmune encephalomyelitis
Cossu D.;
2019-01-01
Abstract
Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in ruminants, has been linked as a possible risk factor for human multiple sclerosis. In the current study we investigated the adjuvant effect of MAP on experimental autoimmune encephalomyelitis (EAE). Groups of C57BL/6 mice were actively immunized with myelin oligodendrocyte glycoprotein (MOG) 35–55 peptide emulsified in incomplete Freund's adjuvant modified containing heat-killed MAP (MIFA). MOG-MIFA immunized mice showed an early disease onset and more severe clinical scores in comparison with MOG-CFA immunized mice, demonstrating for the first time the adjuvant effect of MAP on EAE development.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
