Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacialentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its antiinflammatory activity and safety. Methods: PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells. Results: According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose. Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida,.
The pharmaceutical ability of Pistacia lentiscus L. Leaves essential oil against periodontal bacteria and Candida sp. and its anti-inflammatory potential / Milia, E.; Usai, M.; Szotakova, B.; Elstnerova, M.; Kralova, V.; D'Hallewin, G.; Spissu, Y.; Barberis, A.; Marchetti, M.; Bortone, A.; Campanella, V.; Mastandrea, G.; Langhansova, L.; Eick, S.. - In: ANTIBIOTICS. - ISSN 2079-6382. - 9:6(2020), p. 281. [DOI: 10.3390/antibiotics9060281]
The pharmaceutical ability of Pistacia lentiscus L. Leaves essential oil against periodontal bacteria and Candida sp. and its anti-inflammatory potential
Milia E.
Data Curation
;Usai M.Membro del Collaboration Group
;D'hallewin G.Membro del Collaboration Group
;Spissu Y.Membro del Collaboration Group
;Marchetti M.Membro del Collaboration Group
;Bortone A.Membro del Collaboration Group
;Mastandrea G.;
2020-01-01
Abstract
Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacialentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its antiinflammatory activity and safety. Methods: PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells. Results: According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose. Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida,.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.