Since its discovery, Deinoccocus radiodurans bacterium (Dr) has emerged as one of the most radioresistant microorganism, which can withstand radiation doses greater than 10 KGy, about 3000 times higher than the lethal dose in humans. The understanding of the cell protection ability from radiation-induced death and the high antioxidant capacity opens several potential applications in the field of medicine and biotechnology, as a remedy and cure against radiation injury, in delaying aging and preventing cancer, or for bioremediation of contaminated sites. The mechanism of Dr defence, though not clearly interpreted, seems to involve Mn(II) interaction with ROS-scavenging enzymes or ROS-scavenging complexes with non-enzymatic small-molecule comprising carotenoids, amino acids, peptides and phosphate groups that are crucial for protein protection from oxidative damage during irradiation and, consequently, for Dr survival. The presentation will focus on the role of Mn(II)-peptides and -proteins complexes in the radioresistance mechanism of Dr, by using both a spectroscopic (in vitro) and bioinformatic (in silico) approach [1,2]. References: [1] Peana, M.; Medici, S.; Pangburn, H. A.; Lamkin, T. J.; Ostrowska, M.; Gumienna- Kontecka, E.; Zoroddu, M. A., Manganese binding to antioxidant peptides involved in extreme radiation resistance in Deinococcus radiodurans. Journal of inorganic biochemistry 2016,164, 49-58. [2] Peana, M.; Chasapis, C. T.; Simula, G.; Medici, S.; Zoroddu, M. A., A Model for Manganese interaction with Deinococcus radiodurans proteome network involved in ROS response and defense. Journal of Trace Elements in Medicine and Biology 2018. DOI: 10.1016/j.jtemb.2018.02.001
The role of Mn(II) in the radioresistance mechanism of Deinococcus radiodurans bacterium / Peana, Massimiliano Francesco; Medici, Serenella; Zoroddu, Maria Antonietta. - (2018), pp. 49-49. (Intervento presentato al convegno XIV International Symposium on Inorganic Biochemistry. Science must go on! tenutosi a Wroclaw, Poland nel 5-8 September 2018).
The role of Mn(II) in the radioresistance mechanism of Deinococcus radiodurans bacterium
Massimiliano PEANA;Serenella MEDICI;Maria Antonietta ZORODDU
2018-01-01
Abstract
Since its discovery, Deinoccocus radiodurans bacterium (Dr) has emerged as one of the most radioresistant microorganism, which can withstand radiation doses greater than 10 KGy, about 3000 times higher than the lethal dose in humans. The understanding of the cell protection ability from radiation-induced death and the high antioxidant capacity opens several potential applications in the field of medicine and biotechnology, as a remedy and cure against radiation injury, in delaying aging and preventing cancer, or for bioremediation of contaminated sites. The mechanism of Dr defence, though not clearly interpreted, seems to involve Mn(II) interaction with ROS-scavenging enzymes or ROS-scavenging complexes with non-enzymatic small-molecule comprising carotenoids, amino acids, peptides and phosphate groups that are crucial for protein protection from oxidative damage during irradiation and, consequently, for Dr survival. The presentation will focus on the role of Mn(II)-peptides and -proteins complexes in the radioresistance mechanism of Dr, by using both a spectroscopic (in vitro) and bioinformatic (in silico) approach [1,2]. References: [1] Peana, M.; Medici, S.; Pangburn, H. A.; Lamkin, T. J.; Ostrowska, M.; Gumienna- Kontecka, E.; Zoroddu, M. A., Manganese binding to antioxidant peptides involved in extreme radiation resistance in Deinococcus radiodurans. Journal of inorganic biochemistry 2016,164, 49-58. [2] Peana, M.; Chasapis, C. T.; Simula, G.; Medici, S.; Zoroddu, M. A., A Model for Manganese interaction with Deinococcus radiodurans proteome network involved in ROS response and defense. Journal of Trace Elements in Medicine and Biology 2018. DOI: 10.1016/j.jtemb.2018.02.001I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.