Abstract Ni(II) stimulates innate immunity via the direct binding to human Toll Like Receptor 4 (hTLR4), the bacterial lypopolysaccharide receptor. The binding is specific for humans and causes nickel contact allergy. The protein sequence analysis of hTLR4 revealed that the ectodomain, the region supposed to coordinate the metal ions, contains a histidine-rich motif that is not conserved among all organisms. To elucidate the role of each histidine residue on the protein–nickel binding, we examined the formation of Ni(II) complexes with the model peptide NH2-FQHSNRKQMSERSVFRSRRNRIYRDISHTHTR-COO−, which encompasses the sequence 429–460 of hTLR4. The amino acid sequence of the peptide has been modified by the substitution of some selected lipophilic residues (Leu and Phe) with hydrophilic residues (Arg), aiming at increasing the peptide hydro solubility of the protein fragment. Potentiometric, ultraviolet-visible (UV-Vis), nuclear magnetic resonance (NMR) and circular dichroism (CD) measurements demonstrate that the non-conserved histidines in the ectodomain cooperate in metal coordination and consequently enable the activation of the molecular mechanism of nickel hypersensitivity reaction.

Ni(II) interaction with a peptide model of the human TLR4 ectodomain / Peana, Massimiliano Francesco; Karolina, Zdyb; Medici, Serenella; Pelucelli, Alessio; Giancarlo, Simula; Elzbieta, Gumienna Kontecka; Zoroddu, Maria Antonietta. - In: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY. - ISSN 0946-672X. - 44:(2017), pp. 151-160. [10.1016/j.jtemb.2017.07.006]

Ni(II) interaction with a peptide model of the human TLR4 ectodomain

PEANA, Massimiliano Francesco
;
MEDICI, Serenella;PELUCELLI, Alessio;ZORODDU, Maria Antonietta
2017-01-01

Abstract

Abstract Ni(II) stimulates innate immunity via the direct binding to human Toll Like Receptor 4 (hTLR4), the bacterial lypopolysaccharide receptor. The binding is specific for humans and causes nickel contact allergy. The protein sequence analysis of hTLR4 revealed that the ectodomain, the region supposed to coordinate the metal ions, contains a histidine-rich motif that is not conserved among all organisms. To elucidate the role of each histidine residue on the protein–nickel binding, we examined the formation of Ni(II) complexes with the model peptide NH2-FQHSNRKQMSERSVFRSRRNRIYRDISHTHTR-COO−, which encompasses the sequence 429–460 of hTLR4. The amino acid sequence of the peptide has been modified by the substitution of some selected lipophilic residues (Leu and Phe) with hydrophilic residues (Arg), aiming at increasing the peptide hydro solubility of the protein fragment. Potentiometric, ultraviolet-visible (UV-Vis), nuclear magnetic resonance (NMR) and circular dichroism (CD) measurements demonstrate that the non-conserved histidines in the ectodomain cooperate in metal coordination and consequently enable the activation of the molecular mechanism of nickel hypersensitivity reaction.
2017
Ni(II) interaction with a peptide model of the human TLR4 ectodomain / Peana, Massimiliano Francesco; Karolina, Zdyb; Medici, Serenella; Pelucelli, Alessio; Giancarlo, Simula; Elzbieta, Gumienna Kontecka; Zoroddu, Maria Antonietta. - In: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY. - ISSN 0946-672X. - 44:(2017), pp. 151-160. [10.1016/j.jtemb.2017.07.006]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/181024
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